牛蒡子苷元对ConA诱导小鼠急性肝炎的保护作用  

PROTECTIVE EFFECT OF ARCTIGENIN AGAINST CONCANAVALIN A-INDUCED ACUTE HEPATITIS IN MICE

作　　者：付兴芹 张惠[2] 张青青[2] 姚潇颖 朱玉贞 司传平[2] FU Xingqin;ZHANG Hui;ZHANG Qingqing;YAO Xiaoying;ZHU Yuzhen;SI Chuanping(Department of Immunology,Medical College of Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛大学基础医学院免疫学系,山东青岛266071 [2]济宁医学院免疫学与分子医学研究所

出　　处：《青岛大学学报（医学版）》2018年第3期268-272,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金资助项目(81671632)

摘　　要：目的研究牛蒡子苷元(ATG)对刀豆蛋白A(ConA)诱导的小鼠急性肝炎的保护作用。方法将C57BL/6小鼠随机分成DMSO组、ConA/DMSO组、ConA/ATG组,每组10只小鼠。DMSO组和ConA/DMSO组小鼠腹腔注射DMSO,ConA/ATG组小鼠腹腔注射等体积ATG(30 mg/kg),5h后ConA/DMSO组和ConA/ATG组尾静脉注射ConA(20mg/kg)。12h后处死小鼠,取血清和肝脏组织,用生化分析仪测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平,苏木精-伊红(HE)染色检测肝组织病理损伤,TUNEL染色检测肝细胞凋亡,ELISA方法检测血清肿瘤坏死因子α(TNF-α)和干扰素-γ(IFN-γ)水平,q-PCR方法检测肝组织中TNF-α和IFN-γmRNA表达水平,流式细胞术检测骨髓来源抑制细胞(MDSCs),生化法检测肝脏匀浆丙二醛(MDA)和髓过氧化物酶(MPO)的表达水平。结果与DMSO组相比,ConA/DMSO组血清ALT和AST水平明显升高(F=125.80、94.74,P<0.05);肝脏组织损伤明显加重;肝细胞凋亡增加;血清和肝组织中TNF-α和IFN-γ水平升高(F=17.52、68.81,P<0.05);肝脏MDSCs募集活化降低;同时MDA和MPO活性升高(F=13.38、14.78,P<0.05)。与ConA/DMSO组比较,ConA/ATG组血清ALT和AST水平明显降低(F=125.80、94.74,P<0.05);肝脏组织损伤明显减轻;肝细胞凋亡减少;血清中TNF-α和IFN-γ的水平降低(F=17.52、68.81,P<0.05);肝组织中TNF-α和IFN-γmRNA的表达水平降低(F=31.55、14.64,P<0.05),MDSCs募集活化明显增强;MDA和MPO活性降低(F=13.38、14.78,P<0.05)。结论 ATG对ConA诱导的小鼠急性肝炎具有明显的保护作用。Objective To investigate the protective effect of arctigenin（ATG）against concanavalin A（ConA）-induced acute hepatitis in mice. Methods C57 BL/6 mice were randomly divided into DMSO group,ConA/DMSO group,and ConA/ATG group,with 10 mice in each group.The mice in the DMSO group and the ConA/DMSO group were given intraperitoneal injection of DMSO,and those in the ConA/ATG group were given intraperitoneal injection of the same volume of ATG（30 mg/kg）;5 hlater,the mice in the ConA/DMSO group and the ConA/ATG group were given tail vein injection of ConA（20 mg/kg）.The mice were sacrificed 12 hlater,and serum and liver tissue samples were collected.An automatic biochemical analyzer was used to measure the serum levels of alanine aminotransferase（ALT）and aspartate aminotransferase（AST）;HE staining was used to observe liver pathological injury;TUNEL staining was used to measure cell apoptosis;ELISA was used to measure the serum levels of tumor necrosis factor-α（TNF-α）and interferon gamma（IFN-γ）;q-PCR was used to measure the mRNA expression of TNF-αand IFN-γin liver tissue;flow cytometry was used to measure myeloid-derived suppressor cells（MDSCs）;and the biochemical method was used to measure the expression of malondialdehyde（MDA）and myeloperoxidase（MPO）in liver homogenate. Results Compared with the DMSO group,the ConA/DMSO group had significant increases in the serum levels of ALT and AST（F=125.80 and 94.74,P〈0.05）,significantly greater liver injury,significant increases in hepatocyte apoptosis and the levels of TNF-αand IFN-γin serum and liver tissue（F=17.52 and 68.81,P〈0.05）,a significant reduction in recruitment of MDSCs in the liver,and significant increases in the activities of MDA and MPO（F=13.38 and 14.78,P 0.05）.Compared with the ConA/DMSO group,the ConA/ATG group had significant reductions in the serum levels of ALT and AST（F=125.80 and 94.74,P〈0.05）,significant alleviation of liver injury,a significant reduction in hepatocyte apoptosis,signif

关 键 词：牛蒡子苷元 肝炎 刀豆蛋白A 肿瘤坏死因子Α 干扰素Γ 

分 类 号：R575.1[医药卫生—消化系统]