MicroRNA-200c在结直肠癌中的表达及对侵袭和转移的影响  

Expression of MicroRNA-200c in Colorectal Carcinomas and Its Role on Tumor Cell Migration and Invasion

作　　者：叶伟标[1,2] 徐咏强 李妤玲 卢碧燕 杨湘玲 刘焕亮[2,3] 李仲均 YE Wei-biao;XU Yong-qiang;LI Yu-ling;LU Bi-yan;YANG Xiang-ling;LIU Huan-liang;LI Zhong-jun(Donggnan Hospital of Southern Medical University, Dongguan 523059, China;Guangdong Provincial Key LaboratolT of Colorectal and Pelvic Floor Diseases, Guangzhou 510655, China;The Sixth Affiliated Hospital, Sun Yat sen University, Guangzhou 510655, China.)

机构地区：[1]南方医科大学附属东莞人民医院,广东东莞523059 [2]广东省结直肠盆底疾病研究重点实验室,广东广州510655 [3]中山大学附属第六医院,广东广州510655

出　　处：《中山大学学报（医学版）》2018年第3期335-340,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金青年科学基金(81702399);广东省医学科研基金(C2017034)

摘　　要：【目的】探讨microRNA-200c(miR-200c)在结直肠癌中的表达及其对SW620肠癌细胞株侵袭和转移能力的影响。【方法】采用实时荧光定量PCR检测45例结直肠癌组织和配对邻近正常肠粘膜组织中miR-200c的表达水平;利用脂质体将miR-200c模拟物瞬时转染SW620细胞株,通过Western blot检测上皮间质转化相关蛋白E-cadherin和Vimentin的表达;CCK-8试剂盒和Transwell小室装置检测上调miR-200c的表达对SW620细胞增殖、侵袭和迁移能力的影响。【结果】miR-200c在伴有淋巴结转移的结直肠癌组织中的表达明显下调。体外细胞实验显示,SW620细胞株在转染miR-200c模拟物后,ZEB1蛋白表达受抑制,上皮表型标志物E-cadherin表达升高,而间质表型标志物Vimentin表达下降;上调miR-200c表达可抑制SW620细胞增殖、侵袭和迁移。【结论】miR-200c低表达与结直肠癌侵袭和转移相关,其机制可能是通过调控上皮间质转化过程实现的。【Objective】 To investigate the expression of microRNA-200 c（miR-200 c） in colorectal carcinomas（CRC）,and analyze its role on tumor cell migration and invasion.【Methods】The expression levels of miR-200 c in CRC tissues and adjacent normal mucosa were assessed by real-time quantitative RT-PCR（q RT-PCR）. miR-200 c mimics were transiently transfected into human colorectal cancer cells,and their roles on cell migration and invasion were analyzed by Transwell assay. Cell proliferation was measured using the Cell Counting kit-8. The expression levels of epithelial and mesenchymal markers as well as related transcription factor ZEB1 were detected by Western blotting.【Results】Lower miR-200 c expression was found in primary CRC tissues with lymph node metastasis compared to those without lymph node metastasis and adjacent normal mucosa. Transfection of miR-200 c mimics suppressed proliferation,and reduced invasion and migration in SW620 cells. Furthermore,up-regulation of miR-200 c inhibited ZEB1,and resulted in increased E-cadherin and reduced Vimentin gene expression.【Conclusion】miR-200 c was associated with invasiveand metastatic behavior of CRC. These effects may be mediated through regulation of epithelial-mesenchymal transition.

关 键 词：microRNA-200c 结直肠癌 肿瘤转移 上皮间质转化 

分 类 号：R735.3[医药卫生—肿瘤]