RNA干扰抑制细胞外基质COL6A1基因表达对肾癌786-0细胞增殖和侵袭的影响  

Targeting COL6A1 by RNA interference inhibits proliferation and invasion of renal urothelial carcinoma in 786-0 cells

作　　者：刘世敏[1] 刘述成 李清[1] LIU Shimin;LIU Shucheng;LI Qing(Department of Urology,the First Affiliated Hospital,University of South China,Hengyang 421001, Hunan, China)

机构地区：[1]南华大学附属第一医院泌尿外科,湖南衡阳421001

出　　处：《中南医学科学杂志》2018年第3期276-280,共5页Medical Science Journal of Central South China

摘　　要：研究小干扰RNA(siRNA)抑制COL6A1基因表达对肾癌786-0细胞增殖和侵袭能力的影响。设计合成靶向COL6A1基因的siRNA,瞬时转染肾癌细胞株786-0,利用实时定量聚合酶链反应(qRT-PCR)和Western blot检测RNA干扰后COL6A1基因沉默效果,在RNA干扰后1、2、3天应用噻唑蓝(MTT)比色法检测细胞增殖;应用transwell小室侵袭实验检测肾癌细胞侵袭能力。靶向COL6A1的siRNA成功抑制肾癌细胞株786-0中COL6A1信使RNA(mRNA)及蛋白表达(P<0.05),经MTT测定,肾癌细胞生长受到抑制(P<0.05);transwell小室侵袭实验表明,COL6A1被抑制后,肾癌穿膜细胞数明显减少,侵袭力降低(P<0.05)。应用siRNA技术可有效地抑制COL6A1基因的表达,抑制786-0细胞的体外增殖和侵袭。The aim of this study was to investigate effects of COL6 A1 down-regulation by RNA interference（ RNAi）on proliferation and invasion of renal carcinoma cell line 786-0. SiRNAs targeting COL6 A1 gene were designed,and transiently transfected into renal carcinoma cell line 786-0. The transcription level of COL6 A1 was detected by RT-PCR. Activities of COL6 A1 protein were measured by Western blotting. The cell proliferation was detected by MTT at day 1,day 2,and day 3 after transfection. The cell’s invasion ability was performed on Matrigel transwell assay. The results showed that the levels of COL6 A1 mRNA and protein was significantly reduced after RNAi（ P〈0.05）. MTT assay showed that the proliferation ability of 786-0 cell was decreased due to RNA interference（ P〈0.05）. Transwell assay showed that invasion ability of 786-0 cell was reduced obviously due to COL6 A1 RNAi（ P〈 0.05）. The ability of proliferation and invasion in 786-0 cells can be inhibited by RNAi-targeting COL6 A1.

关 键 词：肾癌 COL6A1 增殖 侵袭 RNA干扰 

分 类 号：R737.11[医药卫生—肿瘤]