Recent advances in enzyme-mediated peptide ligation  

作　　者：Silin Xu Zhenguang Zhao Junfeng Zhao 

机构地区：[1]Key Laboratory of Chemical Biology of Jiangxi Province,College of Chemistry and Chemical Engineering,Jiangxi Normal University [2]The Institute of Chemistry,The Hebrew University of Jerusalem [3]State Key Laboratory of Elemento-Organic Chemistry,Nankai University

出　　处：《Chinese Chemical Letters》2018年第7期1009-1016,共8页中国化学快报（英文版）

基　　金：The National Natural Science Foundation of China (Nos. 21462023, 21778025);the Education Department of Jiangxi Province (No.150297)

摘　　要：Artificial synthesis and site-specific modification of peptides and proteins has evolved into an indispensable tool for protein engineers and chemical biologists. Chemical and enzymatic approaches to peptide ligation are important alternatives of recombinant DNA technology for protein synthesis and modification. Although as old as that of chemical procedures, enzyme-mediated peptide ligation is far less developed than that of chemical counterpart due to the difficult availability of peptide ligase.Fortunately, this situation has been changed slowly with the fast development of biological techniques. In the past decades, several natural peptide ligases have been discovered. Protein engineering to improve the ligation efficiencies of the natural peptide ligase and to reverse the functionality of protease provide more powerful peptide ligases. In this review, the advances of enzyme-mediated peptide ligation and their application in protein synthesis and modification will be discussed.Artificial synthesis and site-specific modification of peptides and proteins has evolved into an indispensable tool for protein engineers and chemical biologists. Chemical and enzymatic approaches to peptide ligation are important alternatives of recombinant DNA technology for protein synthesis and modification. Although as old as that of chemical procedures, enzyme-mediated peptide ligation is far less developed than that of chemical counterpart due to the difficult availability of peptide ligase.Fortunately, this situation has been changed slowly with the fast development of biological techniques. In the past decades, several natural peptide ligases have been discovered. Protein engineering to improve the ligation efficiencies of the natural peptide ligase and to reverse the functionality of protease provide more powerful peptide ligases. In this review, the advances of enzyme-mediated peptide ligation and their application in protein synthesis and modification will be discussed.

关 键 词：LIGASE Peptide synthesis Protein engineering Enzyme-mediated peptide ligation Site-specific protein modification 

分 类 号：O629.7[理学—有机化学]