FOXO3a与乳腺浸润性导管癌血管生成的相关性  

Effect of FOXO3a on angiogenesis in breast invasive ductal carcinoma

作　　者：常艳华[1] 梁加贝[1] 周志毅[1] 蔡颖[1] Yan-hua Chang;Jia-bei Liang;Zhi-yi Zhou;Ying Cai(Department of Pathology, Wuxi People’s Hospital afliated to Nanjing Medical University, Wu xi, Jiangsu 214023, China)

机构地区：[1]南京医科大学附属无锡市人民医院病理科,江苏无锡214023

出　　处：《中国现代医学杂志》2018年第15期37-40,共4页China Journal of Modern Medicine

摘　　要：目的探讨转录因子FOXO3a的表达与乳腺浸润性导管癌血管生成的相关性。方法选择该院行手术切除的乳腺浸润性导管癌组织(乳腺癌组)及相应的癌旁正常组织(癌旁组)各102例,用免疫组织化学法(免疫组化)检测FOXO3a在两组中的表达情况,用CD34标记微血管的内皮细胞,测定微血管密度(MVD),并分析FOXO3a在乳腺癌组织细胞质、细胞核的不同定位与MVD的关系。结果 (1)FOXO3a在乳腺癌中的表达(70.59%,72/102)低于癌旁组织(84.31%,86/102)(P<0.05)。FOXO3a在乳腺癌组织细胞核表达的阳性率为(15.69%,16/102),低于癌旁组织(81.37%,83/103)(P<0.05);而在细胞质表达的阳性率(63.73%,65/102)高于癌旁组(14.71%,15/102)(P<0.05);(2)乳腺癌组织中MVD的水平(27.46±5.87)高于癌旁组(17.25±3.61)(P<0.05)。乳腺癌组织细胞核FOXO3a阳性者MVD的水平(23.19±4.00)低于FOXO3a阴性者(28.24±5.84)(P<0.05);细胞质FOXO3a阳性者MVD的水平(26.39±3.12)与FOXO3a阴性者(27.90±4.37)比较,差异无统计学意义(P>0.05)。结论 FOXO3a在细胞核的表达增加可能对乳腺癌组织中微血管的生成起抑制作用。Objectives To investigate the correlation between expression of FOXO3 a and angiogenesis in breast invasive ductal carcinoma. Methods A total of 102 cases of breast invasive ductal carcinoma tissue（cancer group） and nearby normal tissue group（peri-carcinoma group） were involved in this study. Expression of FOXO3 a was detected by immunohistochemistry. CD34 was stained as a marker of microvascular endothelial cells. Microvessel density（MVD） was evaluated and correlation between MVD and FOXO3 a was analyzed. Results Expression of FOXO3 a in breast cancer cells was significantly lower than that of peri-carcinoma group（70.59% vs 84.31%, P〈0.05）. The positive rate of FOXO3 a in nucleus of breast cancercells was decreased（15.69% vs 81.37%, P〈0.05） while that in cytoplasm increased dramatically（63.73% vs 14.71%, P〈0.05） compared with peri-carcinoma group. MVD in breast cancer tissues and cancer tissues with expression of FOXO3 a in nucleus was upregulated obviously when compared with peri-carcinoma group and cancer tissues without expression of FOXO3 a in nucleus, respectively [（27.46 ± 5.87 vs 17.25 ± 3.61, P〈0.05）,（23.19 ± 4.00 vs 28.24 ± 5.84, P〈0.05）, respectively]. MVD in cancer tissues with expression of FOXO3 a in cytoplasm was not significantly different compared with that in cancer tissue cells without expression of FOXO3 a in cytoplasm（26.39 ± 3.12 vs 27.90 ± 4.37, P〉0.05）. Conclusion Expression of FOXO3 a in nucleus of cancer tissue cells plays an important role in angiogenesis inhibition in breast invasive ductal carcinoma.

关 键 词：乳腺 浸润性导管癌 转录因子FOXO3a 微血管密度 

分 类 号：R737.9[医药卫生—肿瘤]