shPLCε通过PPARβ/twist1抑制前列腺癌细胞的迁移和EMT过程  

shPLCε Can Inhibite Cell Migration and EMT of Prostate Cancer Cell via PPARβ/twist1

作　　者：范佳鑫 李罗 牛凌芳 范砚茹[1] 高英英 吴小候[2] 罗春丽[1] Fan Jiaxin;Li Luo;Niu Lingfang;Fan Yanru;Gao Yingying;Wu Xiaohou;Luo Chunli(Key Laboratory of Diagnostics Medicine of Ministry of Education, Chongqing Medical University, Chongqing 400016, China;Department of Urinary Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China)

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016 [2]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《中国细胞生物学学报》2018年第5期665-674,共10页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81072086)资助的课题~~

摘　　要：该研究旨在探讨磷脂酰肌醇特异性磷脂酶C epsilon(phospholipase C epsilon,PLCε)对前列腺癌细胞上皮–间充质转换(epithelial-mesenchymal transition,EMT)及迁移能力的影响。用LV-sh PLCε感染前列腺癌细胞,q-PCR和Western blot检测PLCε、过氧化物酶体增殖物激活受体β(peroxisome proliferator activated receptorβ,PPARβ)、twist1和EMT相关分子m RNA和蛋白质水平,划痕实验和Transwell实验检测细胞迁移能力。结果表明,感染LV-sh PLCε可显著下调PLCε、PPARβ和twist1的m RNA和蛋白质水平,同时降低前列腺癌细胞株PC3和DU145的迁移能力以及EMT过程。而在sh PLCε组细胞中加入PPARβ的激动剂能一定程度逆转PPARβ和twist1的下调,促进细胞的迁移能力和EMT过程;而PPARβ的抑制剂产生相反作用。该研究说明,PLCε可通过PPARβ/twist1影响前列腺癌细胞的迁移能力和EMT过程。To investigate the effects of PLCε（phospholipase C epsilon） on EMT（epithelial-mesenchymal transition） and migration, we first build sh PLCε cell lines by transfecting LV-sh PLCε into prostate caner cell PC3 and DU145. q-PCR, Western blot, wound heling assay and Transwell experiment were used to investigate the effect of PLCε activity, EMT and migration induced by PLCε knockdown in PC3 and DU145. The results showed that sh PLCε could significantly reduce both m RNA and protein levels of PLCε, PPARβ, and twist1. Wound heling assay and Transwell assay showed that the migration ability was remarkably decreased in the sh PLCε group. At the same time, compared with the blank control group and sh NC group, EMT was also decreased in sh PLCε group（P〈0.05）. After adding the PPARβ agonist in sh PLCε group, the expression of PPARβ and twist1 in both m RNA and protein levels, the migration ability and EMT were increased. The expression of PPARβ and twist1, the migration ability and EMT were lower in sh PLCε＋GSK（PPARβ antagonists） group than those in sh PLCε group.

关 键 词：前列腺癌 磷脂酰肌醇特异性磷脂酶C EPSILON TWIST1 EMT 

分 类 号：R737.25[医药卫生—肿瘤]