高效液相色谱-蒸发光散射法测定栀子油中的5种高级脂肪酸  

Determination of five kinds of fatty acids in gardenia oil by high performance liquid chromatography with evaporative light-scatter detector

作　　者：张风波[1] 罗光明[1] 肖日传 李波[1] 谢晶[1] ZHANG Feng-bo;LUO Guang-ming;XIAO Ri-chuan;LI Bo;XIE Jing(School of Pharmacy, Jiangxi University of traditional Chinese Medicine, Nanchang 330004, Chin)

机构地区：[1]江西中医药大学药学院,南昌330004

出　　处：《中华中医药杂志》2018年第7期3078-3081,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家公益性行业科研专项(No.201507002)~~

摘　　要：目的:建立HPLC-ELSD法分析栀子油中亚麻酸、亚油酸、棕榈酸、油酸和硬脂酸5种高级脂肪酸,并测定其含量。方法:色谱柱Agilent ZORBAX XDB-C18(4.6mm×250mm,5μm),流动相:甲醇-1%乙酸水溶液梯度洗脱,流速:1m L/min,柱温:25℃,ELSD参数:载气:氮气,压力:40Psi,漂移管温度70℃,增益:200,进样量:10μL。结果:亚麻酸、亚油酸、棕榈酸、油酸和硬脂酸的回收率均位于为98.5%~100.5%,相对标准偏差(n=6)分别为1.6%、1.2%、0.8%、1.6%、1.1%。结论:该方法简捷、精确、重复性好,适合栀子油中5种高含量脂肪酸成分的含量测定。Objective： To establish HPLC-ELSD method for analyzing 5 fatty acids in gardenia oil, including linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid, and measure the content. Methods： The Agilent ZORBAX XDB-C18 analytical column（4.6 mm×250 mm, 5μm） with methanol-acetic acid aqueous solution as mobile phase at a flow rate of 1.0 mL/min were applied. The column temperature was set at 25℃. The parameters of ELSD were as follows： the carrier gas pressure was 40 Psi, the temperature of the drift tube was 70℃, the plus was 200, sample size was 10μL. Results： The recoveries of linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid were all located at 98.5% to 100.5%, and the relative standard deviation（n=6） was 1.6%, 1.2%, 0.8%, 1.6%, 1.1%. Conclusion： This method is simple, accurated and reproducible, it is suitable for the simultaneous determination of the five kinds of high fatty acids in gardenia oil.

关 键 词：栀子油 高级脂肪酸 高效液相色谱-蒸发光散射 含量测定 

分 类 号：R284.1[医药卫生—中药学]