血管生成素-2在大鼠急性胰腺炎血清中的表达及其意义  

Changes of angiopoietin-2 levels in serum of rats with acute pancreatitis

作　　者：刘隽[1] 王国良[1] 徐贤刚[1] 柳严[1] 程建华[1] 黄建钊[1] Liu Jun;Wang Guoliang;Xu Xiangang;Liu Yan;Cheng Jianhua;Huang Jianzhao(Depatment of Hepatobiliary Surgery, Guizhou Proviencial People＇ s Hospital, Guiyang 550002, Chin)

机构地区：[1]贵州省人民医院肝胆外科,贵阳550002

出　　处：《中华实验外科杂志》2018年第7期1268-1270,共3页Chinese Journal of Experimental Surgery

摘　　要：目的对血管生成素-2（Ang-2）在急性胰腺炎（AP）中的表达及其意义。方法健康SD大鼠40只，随机分为5组：对照组、急性胰腺炎模型组（分为AP6h组、AP12h组、AP24h组、AP48h组），每组8只。AP组分别于建模后6、12、24、48h处死，对照组于第2次注射后24h处死。酶联免疫吸附（ELISA）法检测血清Ang-2、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL-6）水平；提取胰腺组织RNA，实时定量聚合酶链反应（Real-time PCR）测定Ang-2、磷脂酰肌醇-3-激酶（P13K）、蛋白激酶B（PKB、Akt）mRNA表达水平。结果同对照组比较，AP组血清Ang-2水平表达水平明显升高，6h即差异有统计学意义，24～48h达到高峰（P=0．032）；胰腺组织Ang-2、PI3K、Akt mRNA表达水平明显升高（P=0．028），与AP发生时间呈正相关；炎性相关因子TNF-α、IL-6水平明显升高（P=0．026、0．011）。6-24hAP组血清Ang-2水平与胰腺Ang-2mRNA水平表达呈正相关（r=0．392，P=0．034）；P13K、Akt表达水平与Ang-2mRNA水平呈正相关（r=0．432、0．451，P=0．029），血清TNF-α、IL-6表达水平与胰腺Ang-2mRNA水平呈正相关（r=0．412、0．389，P=0．035）。结论血清Ang-2水平在AP发生早期即出现明显升高，持续48h以上，是协助急性胰腺炎诊断的敏感指标；Ang-2可激活P13K／Akt信号通路，也可能是AP过程中TNF-α及IL-6水平升高的原因之一。Objective To investigate dynamic changes of angiopoietin - 2 （ Ang - 2 ） in acute pancreatitis （AP） and its significance, Methods Forty healthy SD rats were randomly divided into 5 groups： control group, acute pancreatitis model group （AP 6 h group, AP 12 h group, AP 24 h group and AP 48 h group）. AP group were killed at 6, 12, 24 and 48 h after modeling. The control group was killed after the second injection of 24 hours. The levels of serum Ang - 2, tumor necrosis factor - α （ TNF - α） , interleukin （IL- 6） were detected by enzyme -linked immuuosorbentassay （ELISA）. The expression of phosphatidylinositol -3 -hydroxy kinase （PI3K） and protein kinase B （PKB, Akt） mRNA expression levels were detected by Real - time polymerase chain reaction. Results The serum level of Ang - 2 in the AP group was significantly higher than that in the control group（P =0. 032）. The expression of PI3K/Akt mRNA in pancreatic tissue of AP group was significantly higher than that in the control group （P = 0. 028 ） , and the levels of TNF - α and IL - 6 were significantly increased （ P = 0. 026, 0. 011 ）. The level of Ang - 2 in 6 - 24 h was positively correlated with Ang - 2 mRNA in pancreas （ r = 0. 392, P = 0. 034）. The expression of PI3K and Akt was positively correlated with Ang -2 mRNA level （r = 0. 432, 0. 451, P = 0. 029） and the levels of serum TNF - α and IL - 6 were positively correlated with Ang - 2 mRNA levels in pancreas （ r = 0. 412, 0. 389, P = 0. 035）. Conclusion Serum Ang - 2 level of AP groupis significandy higher than that of the control group. Aug- 2 can activate PI3K/Akt signaling pathway and may he one of the reasons of high TNF - α and IL - 6 levels in AP. SerumAng - 2 level deteetionmay provide the basis for early assessment of the severity of acute panereatitis.

关 键 词：急性胰腺炎 血管生成素-2 磷脂酰肌醇-3-激酶/蛋白激酶B 血清学检测 

分 类 号：R576[医药卫生—消化系统]