腺相关病毒介导的趋化因子受体4短发卡RNA对非小细胞肺癌裸鼠肿瘤生长的影响  

Effect of CXC chemokine receptor 4 short hairpin RNA mediated by recombinant adenovirus vector on tumor growth in nude mice with non small cell lung cancer

作　　者：刘青锋[1] 杨军峰[1] 侯广杰[1] 董冠中[1] 赵璞[1] 杨光煜[1] 何苡[1] Liu Qinjeng;Yang Junfeng;Hou Guangjie;Dong Guanzhong;Zhao Pu;Yang Guangyu;He Yi(Department of Chest Surgery, Henan Provincial People＇ s Hospital, Zhengzhou 450003, Chin)

机构地区：[1]河南省人民医院胸外科,郑州450003

出　　处：《中华实验外科杂志》2018年第7期1274-1276,共3页Chinese Journal of Experimental Surgery

基　　金：2015年河南省医学科技攻关计划项目（201503180）

摘　　要：目的探讨腺相关病毒介导的趋化因子受体4（CXCR4）短发卡RNA（shRNA）对非小细胞肺癌裸鼠肿瘤生长的抑制作用。方法采用腺相关病毒介导的CXCR4 shRNA抑制人非小细胞肺癌A549细胞中CXCR4的表达，采用实时荧光定量聚合酶链反应（FQ-PCR）检测A549细胞中CXCR4 mRNA的表达水平；建立人非小细胞肺癌A549的裸鼠肿瘤模型，分为空病毒组和CXCR4 shRNA组，记录两组裸鼠肿瘤体积、质量、数量和生存期，计算裸鼠生存延长率和肿瘤抑制率；取死产后裸鼠肿瘤组织，采刖Western blot法检测肿瘤组织中血管内皮生长因子（VEGF）的蛋向表达水平。结果与空病毒组（1．17±0．19）比较，重组腺病毒载体感染后的A549细胞CXCR4 mRNA表达水平（0．26±0．10）显著下调，差异有统计学意义（P=0．000）。CXCR4-shRNA组裸鼠肿瘤平均体积、平均质量和平均数量，显著低于空病毒组，差异有统计学意义（P=0．000）。CXCR4-shRNA组平均生存时间显著长于空病毒组（P=0．000）；CXCR4-shRNA组的生存延迟率和肿瘤抑制率显著高于空病毒组（P均=0．000），CXCR4-shRNA组VEGF蛋白表达水平（0．28±0．05）显著低于空病毒组（1．02±0．23），差异有统计学意义（P=0．000）。结论腺相关病毒介导的CXCR4 shRNA能够抑制非小细胞肺癌裸鼠肿瘤的生长及转移。Objective To investigate the inhibitory effect of CXC chemokine receptor 4 （CXCR4） short hairpin RNA （shRNA） mediated by recombinant adenovirus vector on tumor growth in nude mice with non small cell lung cancer. Methods Inhibiting the expression of CXCR4 in non - small cell lung cancer cells A549 using CXCR4 shRNA mediated by recombinant adenovirus vector. The expression of CXCR4 mRNA in A549 cells were detected by real - time fluorescent quantitative polymerase chain reaction （ FQ - PCR） method. Two groups of nude mice tumor model with non small cell lung eancer were established： the CXCR4 mRNA group and the empty virus group. The tumor volume, quality, quantity and survival time were recorded. The survival rate and tumor inhibition rate of nude mice in two groups were calculated. The expression levels of vascular endothelial growth factor （VEGF） protein in tumor tissue of mice after death was detected by Western blotting method. Results Compared with the empty virus group （1. 17 ±0. 19） , the expression level of CXCR4 mRNA in A549 cells infected by recombinant adenovirus vector （0. 26 ±0. 10） significantly decreased （P =0. 000）. The average volume, mean mass and average number of tumor in group CXCR4 - shRNA were significantly lower than that of the nude virus group, （P = 0. 000）. The CXCR4 - shRNA group the average survival time was significantly longer than the empty virus group （P =0. 000） ; CXCR4 -shRNA group survival rate and delayed tumor growth inhibition rate was significantly higher than that of the empty virus group （ P = 0. 000 ） , CXCR4 - shRNA group, VEGF protein expression level （0. 28 ± 0. 05 ） was significantly lower than that of the empty virus group （ 1.02 ± 0.23 ） , the difference was statistically significant （P = 0. 000）. Conclusion CXCR4 shRNA mediated by recombinant adenovirus vector can inhibit the growth and metastasis in nude mice with non small cell lung cancer.

关 键 词：趋化因子受体4短发卡RNA 非小细胞肺癌 裸鼠 肿瘤抑制 

分 类 号：R734.2[医药卫生—肿瘤]