转基因抗虫水稻秀水134-Bt毒蛋白的时空表达分析  

Temporal and spatial expression of Bt toxic protein in transgenic insect-resistant rice Xiushui 134-Bt

作　　者：白建江[1] 张建明[1] 朴钟泽[1] 方军[1] 杨瑞芳[1,2] 李钢燮 BAI Jian-jiang;ZHANG Jian-ming;PIAO Zhong-ze;FANG Jun;YANG Rui-fang;LEE Gang-seob(Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Shanghai Nongke Seeds and Seedlings Company Limited, Shanghai 201106, China;National Academy of Agricultural Science （ South Korea）, Suwon City 441-857, South Korea)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]上海农科种子种苗有限公司,上海201106 [3]韩国农村振兴厅农业技术学院

出　　处：《上海农业学报》2018年第3期50-53,共4页Acta Agriculturae Shanghai

基　　金：沪农科种字(2016)第1-4号;上海市青年科技启明星计划(B类)(16QB1402500);农科创2017A05;中韩国际合作项目(PJ013647)

摘　　要：采用双抗夹心酶联免疫(ELISA)方法研究了转基因抗虫水稻秀水134-Bt不同生育期和不同组织中Bt毒蛋白的表达情况。结果表明:秀水134-Bt毒蛋白在各个组织中均有表达,但不同器官中表达量明显不同。Bt毒蛋白在叶片中表达量最高,叶鞘次之,根、茎、小穗中表达量相对较低,成熟种子中几乎检测不到。不同发育期叶片Bt毒蛋白的表达有显著差异,Bt毒蛋白表达量随着植株的生长而减少。研究结果可为转基因抗虫水稻适宜检测时间选择提供一定的参考。The expression of Bt toxin protein in different growth stages and tissues of Xiushui 134-Bt wasstudied by double antibody sandwich ELISA. The results showed that Bt toxin protein of Xiushui 134-Bt wasexpressed in all tissues,but the expression level was different in different organs. The expression level of Btprotein in leaves was the highest,followed by leaf sheath. The expression level in roots,stems and spikelets wasrelatively low,and hardly detected in mature seeds. The expression of Bt toxin protein was significantly differentat different growth stages,and decreased with plant growth. The results could provide a reference for selectingsuitable detection time for transgenic insect-resistant rice.

关 键 词：水稻 秀水134-Bt BT毒蛋白 时空表达 双抗夹心酶联免疫 

分 类 号：S511.032[农业科学—作物学]