PinX1基因对黑色素瘤细胞迁移和侵袭能力的影响及机制研究  

The effect and mechanism of PinX1 on migration and invasion of melanoma cells

作　　者：赵杨 郑雨晴 章宏伟[2] Zhao Yang;Zheng Yuqing;Zhang Hongwei(Department of Plastic and Burn Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China)

机构地区：[1]南京医科大学第一附属医院整形烧伤科,210029 [2]南京医科大学附属友谊整形外科医院整形外科,210029

出　　处：《中华整形外科杂志》2018年第6期483-487,共5页Chinese Journal of Plastic Surgery

摘　　要：目的研究PinXl基因对黑色素瘤细胞迁移和侵袭能力的影响，并初步探讨其与细胞外基质的相关机制。 方法将PinX1-siRNA分别转染至黑色素瘤细胞A375和MV3中，运用CCK8增殖实验检测PinXl基因对于黑色素瘤细胞增殖能力的影响；通过Transwell实验观察PinX1基因对黑色素瘤细胞迁移和侵袭能力的改变；使用Western Blot测定PinXl、MMP-9和TIMP-1的蛋白表达水平。 结果下调PinX1表达后，黑色素瘤细胞的增殖、迁移和侵袭能力增强，基质金属蛋白酶MMP-9蛋白的表达水平显著增高（P〈0.01），TIMP-l蛋白水平明显降低（P〈0.01）。 结论在黑色素瘤细胞中低表达PinXl，可能通过下调TIMP-1蛋白引起MMP-9通路激活，进而提高黑色素瘤细胞的迁移和侵袭能力。ObjectiveTo explore the role of PinXl in the migration and invasion of melanoma cells, and study the mechanism from the perspective of extracellular matrix . MethodsPinX1-siRNA was transfected into melanoma cells A375 and MV3 respectively. Detect the effect of PinXl on proliferation abilities of melanoma cells A375 and MV3 by CCK-8 assay. The migration and invasion abilities of the two cell lines were detected by Transwell migration and invasion assay experiments.The protein expression levels of PinXl, TIMP-1 and MMP-9 were detected by Western Blot analysis. ResultsThe proliferation, migration and invasion abilities of melanoma cells A375 and MV3 were significantly increased after PinX1 gene knockdown. The expression level of MMP-9 was significantly increased （P〈0.01） and the expression level of TIMP-l was reduced significantly（P〈0.01）. ConclusionsDown-regulation of PinX1 may activate the MMP-9 pathway by down-regulating the TIMP-1 protein, which improve the migration and invasion ability of melanoma cells.

关 键 词：黑色素瘤 迁移 侵袭 基因 

分 类 号：R739.5[医药卫生—肿瘤]