六君子汤含药血清对TGF-β1介导的A549细胞MMP-9和整合素β1蛋白表达的影响  

Effect of Serum Containing Liujunzi Decoction on MMP-9 and Integrinβ1 of A549 Cells Mediated by TGF-β1

作　　者：姜林林[1] 于丹[1] 唐莹[1] 刘春英[1] JIANG Linlin;YU Dan;TANG Ying;LIU Chunying(Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China)

机构地区：[1]辽宁中医药大学

出　　处：《中华中医药学刊》2018年第7期1601-1604,I0007,I0008,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473569)

摘　　要：目的：观察六君子汤含药血清对TGF-β1介导的A549细胞MMP-9和整合素β1表达的影响,探讨六君子汤影响肺癌转移的可能机制。方法：制备六君子汤含药血清,体外培养A549细胞。实验分三组：空白血清组,空白血清＋TGF-β1组,六君子汤最佳浓度含药血清＋TGF-β1组。普通显微镜下观察TGF-β1对A549细胞的干预程度。细胞划痕实验检测六君子汤含药血清对TGF-β1介导的A549细胞迁移能力的影响。免疫细胞化学法和Western Blot技术检测MMP-9和整合素β1在蛋白表达水平上的变化。结果：TGF-β1能够明显诱导A549细胞发生上皮间质转化。与空白血清组比较,空白血清＋TGF-β1组细胞迁移程度明显增加（P〈0.05）,MMP-9和整合素β1的蛋白表达增加（P〈0.05）;与空白血清＋TGF-β1组比较,含药血清组细胞迁移程度下降（P〈0.05）,MMP-9和整合素β1的蛋白表达下降（P〈0.05）。结论：（1）六君子汤含药血清能够减缓TGF-β1介导的A549细胞的迁移能力;（2）六君子汤含药血清能够降低TGF-β1介导的A549细胞MMP-9和整合素β1蛋白的表达。Objective： To observe the effects of serum containing Liujunzi Decoction on the expressioons of MMP-9 and Integrinβ1 in cells A549 mediated by TGF-β1 and explore the possible mechanism of affecting lung cancer metastasis. Methods： We prepared the serum containing Liujunzi Decoction and A549 cells were cultured in vitro. The experiment was divided into the blank serum group,blank serum ＋ TGF-β1 groupand the best containing medicine serum ＋ TGF-β1 group. We observed the intervention degree of TGF-β1 on A549 cells under general microscope. The effect of serum containing Liujunzi Decoction on TGF-β1 mediated A549 cells migration was observed by scratch test. The expressions of MMP-9 and Integrinβ1 in protein were detected by immunocytochemical method and Western blot. Results： TGF-β1 can obviously induce epithelial mesenchymal transition of A549 cells. Compared with blank serum group,the degree of cell migration increased significantly in blank serum ＋ TGF-β1 group（ P 〈 0. 05） and the protein expression of MMP-9 and Integrinβ1 increased（ P 〈 0. 05）. Compared with blank serum ＋ TGF-β1 group,the migration degree of drug serum group decreased（ P 〈 0. 05） and the protein expressions of MMP-9 and Integrinβ1 decreased（ P 〈 0. 05）. Conclusion：The serum containing Liujunzi Decoction can slow down the migration of A549 cells mediated by TGF-β1 and can reduce the expressions of MMP-9 and Integrinβ1 protein in A549 cells mediated by TGF-β1.

关 键 词：六君子汤 A549细胞 MMP-9 整合素Β1 EMT 转移 

分 类 号：R734.2[医药卫生—肿瘤]