辛伐他汀对大肠癌细胞系HCT116组织因子表达的影响  

Effects of Simvastatin on the Expression of Tissue Factor in HCT116 Colorectal Cancer cell line

作　　者：聂群 燕瑾 聂李平[2] 谢闻悦[2] 李建新[2] NIE Qun;YAN Jin;NIE Li- ping;XIE Wen- yue;LI Jian- xin(Department of Clinical Laboratory, Dapeng New District Maternal and Children Health Hospital, Shenzhen 518120, China;Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, China)

机构地区：[1]深圳市大鹏新区妇幼保健院检验科,深圳518120 [2]北京大学深圳医院检验科,深圳518036

出　　处：《血栓与止血学》2018年第3期398-401,共4页Chinese Journal of Thrombosis and Hemostasis

基　　金：深圳市科技创新委基础研究资助项目(JCYJ20150403091443282)

摘　　要：目的探索辛伐他汀对大肠癌细胞系HCT116组织因子(TF)表达的影响。方法用实时荧光定量PCR和流式细胞术检测给药前后大肠癌细胞系HCT116的TF m RNA和TF膜蛋白表达含量,并评价其表达水平差异。结果PCR结果显示,相对于空白组,10^(-7)mol/L和10^(-5)mol/L辛伐他汀浓度组TF m RNA的表达倍数表分别为0.693±0.052和0.280±0.021,差异有统计学意义(P<0.05和P<0.01)。流式细胞术的检测结果表明,与空白组(26.25±0.639)比较,10^(-7)mol/L和10^(-5)mol/L辛伐他汀浓度组TF膜蛋白表达的数学平均数分别为19.76±0.550和15.45±0.518,差异非常显著(P均<0.01)。结论辛伐他汀可剂量依赖地下调HCT 116细胞的TF m RNA和TF膜蛋白的表达。ObjectiveTo study the effect of simvastatin on the expression of tissue factor（TF）in colorectal cancer cell line HCT116.MethodsThe expression of TF m RNA and membrane protein in colorectal cancer cell line HCT116 before and after administration of simvastatin was detected by real-time fluorescence quantitative PCR and flow cytometry,respectively.ResultsThe results of PCR showed that,as compared to the blank group,the relative folds of TF m RNA in 10^-7mol/L and 10^-5mol/L simvastatin group was 0.693±0.052 and 0.280±0.021,respectively,and the difference was statistically significant（P0.05and P〈0.01）.The results of flow cytometry showed that,the mathematical mean of TF membrane protein expression in 10^-7mol/Land 10^-5mol/L simvastatin group was 19.76±0.550 and 15.45±0.518,respectively.As compared with the blank group（26.25±0.639）,TF expression values decreased significantly（all P〈0.01）.ConclusionSimvastatin can dose-dependently down-regulate the expression of TF m RNA and membrane protein in HCT116 cells.

关 键 词：辛伐他汀 大肠癌细胞 组织因子 

分 类 号：R361.3[医药卫生—病理学]