液相芯片技术检测癫痫大鼠海马组织RANTES、MCP-1、IP-10的表达研究  

Study on Expressions of RANTES,MCP-1 and IP-10 in Hippocampus of Epileptic Rats by Liquid Chromatography

作　　者：刘倩[1] 黄银兰[1] 薛小卫[1] 李洋洋[1] 蔺耐荣 LIU Qian;HUANG Yinlan;XUE Xiaowei;LI Yangyang;LIN Nairong(Ningxia Medical University, Yinchuan 750004, Ningxia, Chin)

机构地区：[1]宁夏医科大学

出　　处：《辽宁中医杂志》2018年第5期1062-1065,共4页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的：观察趋化因子RANTES、MCP-1、IP-10在癫痫大鼠海马组织中的表达情况,探讨百会穴治疗癫痫的作用机制。方法：将30只200~250 g的雄性SD大鼠随机分为正常空白组、正常百会组、模型空白组、模型百会组。建立氯化锂-匹鲁卡品癫痫大鼠模型,针刺其百会穴。运用液相芯片技术检测癫痫大鼠海马组织中RANTES、MCP-1、IP-10的蛋白表达情况。结果：四组大鼠RANTES、MCP-1、IP-10的表达情况均为：模型空白组的蛋白表达量明显高于正常空白组（P〈0.05）;模型百会组高于正常百会组（P〈0.05）;模型百会组低于模型空白组（P〉0.05）;正常空白组和正常百会组相比（P〉0.05）。结论：大鼠海马组织趋化因子RANTES、MCP-1、IP-10参与了癫痫疾病的发生和发展过程;针刺百会穴未能通过降低RANTES、MCP-1、IP-10的蛋白含量来达到治疗癫痫的目的。Objective： To observe the expressions of chemokines RANTES,MCP-1 and IP-10 in the hippocampus of epilepsy rats and explore the mechanism of Baihui Injection in the treatment of epilepsy. Methods： Thirty male SD rats weighed 200 ~ 250 g were randomly divided into normal control group,normal Baihui（DU20） group,model control group,model Baihui（DU20） group.The model of lithium pilocarpine epilepsy was set up. The expressions of RANTES,MCP-1 and IP-10 in hippocampus of epilepsy rats were detected by liquid chip technique. Results： The expressions of RANTES,MCP-1 and IP-10 in the model control group was significantly higher than those of the normal control group（P〈0. 05）. The model Baihui（DU20） group＇s were higher than those of the normal Baihui（DU20） group（P〈0. 05）. The model Baihui（DU20） group＇s were lower than those of the model control group（P〉0. 05）. The normal control group and normal Baihui（DU20） group had no difference（P〉0. 05）. Conclusion：The chemotactic RANTES,MCP-1 and IP-10 in the hippocampus of rats were involved in the development and progression of epilepsy. The acupuncture at Baihui（DU20） failed to reduce the protein contents of RANTES,MCP-1 and IP-10 to achieve the purpose of treatment of epilepsy.

关 键 词：针刺 癫痫 趋化因子 液相芯片技术 

分 类 号：R742.1[医药卫生—神经病学与精神病学]