国内羊胃肠道寄生虫流行病学调查及捻转血矛线虫系统进化分析  

The epidemical survey of sheep gastrointestinal parasites in partial areas of China

作　　者：郭筱璐 杨怡[1] 施宇 黄艳[1] 时恒枝 张玲 周静茹 陈学秋[1] 杜爱芳[1] GUO Xiao-lu;YANG Yi;SHI Yu;HUANG Yan;SHI Heng-zhi;ZHANG Ling;ZHOU Jing-ru;CHEN Xue-qiu;DU Ai-fang(Zhejiang Provincial Key Laboratory o j Preventive Veterinary Medicine,College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院浙江省动物预防医学重点实验室

出　　处：《中国兽医学报》2018年第7期1332-1337,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372426);国家自然科学青年基金资助项目(31602041)

摘　　要：为更好地掌握我国部分地区羊胃肠道寄生虫病的流行情况以及捻转血矛线虫zJ株近年来的系统进化关系，从而为捻转血矛线虫病的防治提供科学依据，于2014年9-12月采集了来自山东、吉林、黑龙江、内蒙古、辽宁、河北、江苏、天津和青海9个省（市）的49（9份羊粪样品，对其进行了寄生虫虫卵分类鉴定，对羊寄生虫病的流行病学特征进行总结分析；此外实验室于2004-2013年采集了来自浙江省嘉兴地区的15份捻转血矛线虫ZJ株成虫样品，设计特异性引物对样品ITG2和ND4基因进行分子克隆测序鉴定比对，对该虫近10年来的系统进化进行分析。结果表明，全国各地羊体内肠道寄生虫的感染情况均较为严重，其中感染虫种的严重程度依次为原虫（83．27‰）、线虫（64．29％）和绦虫（49．80％），感染相对最为严重的3个地区分别为天津、青海和内蒙古，而相对较轻的3个地区为江苏、黑龙江、山东。对捻转血矛线虫ZJ株的15份样品进行系统进化分析显示，ITS-2和ND4基因在10年间均有不同程度的遗传变异发生，变异范围分别为0．0％-3．9％和0．4‰-4．8％，而ND4突变程度较大可能是由于虫种固有差异引起的。而2种基因都有部分位点突变后与H．placei（Haemonchus pacei）相同，说明嘉兴地区可能存在捻转血矛线虫和H．placei交叉感染的情况。调查分析结果将为制定更合理的羊寄生虫病防控方案提供参考依据，并为寄生虫系统进化分析研究奠定基础。To have a better understanding of the sheep gastrointestinal parasites epidemiology in partial areas of China,490 fecal samples were collected from Shandong,Jilin, Heilongjiang, Nei-menggu, Liaoning, Hebei ,Jiangsu, Tianjin and Qinghai during September to December in 2014 and identified for egg morphology. At the meantime, 15 samples of Haemonchus contortus （H. contorlus） were collected from Jiangxing area of Zhejiang province since 2004 to perform the phyletic e volution analysis using ITS 2 and ND4 as gene markers. As the results showed,severe infection of gastrointestinal parasites could be observed in all these nine areas with the highest infection rate of protozoans（83.27G）,followed by nematodes（64.29%） and tape worms（49. 8%）. The most se- vere areas were Tianjin, Qinghai and Neimenggu. The phyletic evolution analysis indicated that both ITS 2 and ND4 genes showed some level of variation among 0.0%-3.9% and 0.4%-4.8% in the last decade. The relatively high variation of ND4 gene may be caused by the inherent difference of ZJ strain compared to the reference strain. Some sites of these two genes of the 15 samples shared identical variations with Haemonchus pacei ,this may indicate that there was cross-infection with H. contorlus and H. placei in Jiaxing area. This research laid a solid foundation for further study of the control and prevention of sheep gastrointestinal parasites.

关 键 词：胃肠道寄生虫 流行病学调查 捻转血矛线虫 系统进化分析 

分 类 号：Q958.9[生物学—动物学]