2015年-2017年珠海市肠炎沙门菌分子分型和耐药性研究  

Study on molecular typing and drug resistance of Salmonella enteritidis in Zhuhai,2015-2017

作　　者：周兰兰[1] 方艳梅[1] 魏泉德[1] 陈红涛[2] 张晟春 周玉球[4] ZHOU Lan -lan;FANG Yan- mei;WEI Quan - de;CHEN Hong - tao;ZHANG Sheng - chun;ZHOU Yu - qiu(Zhuhai Center for Disease Control and Prevention, Zhuhai, Guangdong 519000, China)

机构地区：[1]珠海市疾病预防控制中心,广东珠海519000 [2]中山大学第五附属医院,广东珠海519000 [3]遵义医学院第五附属医院,广东珠海519000 [4]珠海市妇幼保健院,广东珠海519000

出　　处：《中国卫生检验杂志》2018年第13期1573-1575,1595,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的分析珠海市2015年-2017年腹泻患者肠炎沙门菌的耐药情况和脉冲场凝胶电泳(PFGE)分型特征。方法收集的79份腹泻患者粪便标本,经分离培养、生化和血清鉴定为肠炎沙门菌后,使用药敏纸片法检测菌株对14种抗生素耐药性,同时使用PFGE法进行分子分型。结果共分离获得79株肠炎沙门菌,男、女感染比为1.08∶1,1岁~3岁幼儿感染为44.30%,分离高峰为6月-10月;所有肠炎沙门菌对亚胺培南、头孢西丁、庆大霉素和阿奇霉素均敏感,对氨苄西林/舒巴坦、氨苄西林和萘啶酸耐药率较高,54株菌出现多重耐药;PFGE分型发现79株菌分为26种带型和4个簇,70.89%菌株聚集在B簇,聚类相似性为92.30%;B簇56株菌和79株肠炎沙门菌对氨苄西林耐药性差异有统计学意义(P<0.05)。结论肠炎沙门菌感染无性别差异,以1岁~3岁幼儿感染为主,呈现夏秋季流行特点。珠海市肠炎沙门菌的耐药情况比较严峻,基因型别较多,感染来源广泛。肠炎沙门菌PFGE分子分型条带与氨苄西林耐药有相关性。Objective To analyze the antibiotic resistance and molecular epidemiological characteristics of Salmonella enteritidis isolated from diarrhea patients in Zhuhai during 2015-2017. Methods 79 fecal specimens collected from patients with diarrhea were identified as Salmonella enteritidis after isolation,culture,biochemistry and sera identification. The resistance of the strains to 14 antibiotics was tested using a drug sensitive paper method,and molecular typing was performed using the pulsed-field gel electrophoresis. Results In total,79 strains of Salmonella enteritidis were identified,with the infection rate of male to female as 1. 08∶1. 1-3 year old children accounted for 44. 30%. The isolation peak was from June to October. All strains were sensitive to imipenem,cefoxitin,gentamicin and azithromycin,but showed high resistance to ampicillin,ampicillin/sulbactam and nalidixic acid. There were 54 multidrug resistant strains. All strains were divided into 26 types of PFGE and 4 clusters. 70. 89% strains clustered in clade B,with similarityof 92. 30%. The resistance difference of 56 strains in the clade B and 79 strains of Salmonella enteritidis to ampicillin was statistically significant（ P〈0. 05）. Conclusion The infection of Salmonella enteritidis showed no difference in gender,mainly concentrated in 1-3 year old children and showing prevalence in summer and autumn. The drug resistance of Salmonella enteritidis in Zhuhai was severe. There are many genotypes and a wide sources of infection. Molecular typing of Salmonella enteritidis by PFGE is associated with ampicillin resistance.

关 键 词：肠炎沙门菌 脉冲场凝胶电泳 耐药性 分子分型 

分 类 号：R446.5[医药卫生—诊断学]