恩度微量泵入与腹腔注射联合表阿霉素治疗小鼠乳腺癌的疗效对比观察  

Effect of Two Administration Methods of Endostar Combined with Epirubicin on Breast Cancer in Mice

作　　者：吴皎[1] 杨慧勤 刘蓉 杨润祥[1] WU Jiao;YANG Huiqin;LIU Rong;YANG Runxiang(Department of Internal Medicine, The Third Affiliated Hospital of Kunming Medical University, Yunnan Province Tumor Hospital, Kunming 650118, China;Department of Osteopathic Medicine, Kunming Yan ＂an Hospital, Kunming 650051, China;Key Laboratory of Animal Models and Human Disease Mechanism, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China)

机构地区：[1]昆明医科大学附属第三医院,云南省肿瘤医院内二科,昆明650118 [2]云南省昆明市延安医院骨科,昆明650051 [3]中国科学院昆明动物研究所动物模型与人类疾病机理重点实验室,昆明650223

出　　处：《肿瘤防治研究》2018年第7期463-467,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81360393;81560432);CSCO血管靶向治疗研究基金(S-S2015-001)

摘　　要：目的比较恩度微量泵入或腹腔注射联合表阿霉素对小鼠乳腺癌的疗效。方法建立乳腺癌4T1细胞的BALB/c小鼠移植瘤模型,随机分为空白对照组(0.9%Na Cl,ip)、标准对照组(EPI,ip)及4个实验组:(1)rh ES(ip)+EPI(ip);(2)rh ES(pump)+EPI(ip);(3)rh ES(ip);(4)rh ES(pump)。监测肿瘤大小和小鼠体重,治疗第10天行小动物活体成像,治疗结束后采用免疫组织化学法测定瘤内血小板-内皮细胞黏附分子(CD31)的表达。结果恩度单药持续微量泵入(7 mg/(kg.d))疗效显著优于腹腔注射(P=0.01);恩度持续微量泵入联合表阿霉素与双药联合腹腔注射疗效相当(P>0.05),但前者不良反应略低。结论恩度微量泵入联合表阿霉素治疗小鼠乳腺癌疗效与传统静脉滴注相当,为开展乳腺癌患者携泵回家治疗的可能性提供了实验依据,但还需要进一步的临床试验加以证实。Objective To compare the effect of endostar via micro pump or intraperitoneal injection combined with epirubicin on breast cancer in mice. Methods We established the BALB/c mice breast cancer model by orthotopically inoculating 4T1 cells, then they were randomly divided into six groups： blank group（0.9%NaCl, ip）, standard control group（EPI, ip） and four experimental groups：（1） rhES（ip）＋EPI（ip）; （2） rhES（pump）＋EPI（ip）; （3） rhES（ip）; （4） rhES（pump）. We monitored tumor xenografts volume, mice weight and drug adverse events every two days until the termination of treatment; We also detected their bioluminescence activity on day 10; after the treatment, mices were sacrificed, tumor xenografts were completely removed and we detected platelet endothelial cell adhesion molecule-1 （PECAM-1/CD31） expression level in tumor tissues by IHC. Results The effect of endostar via micro pump（7 mg/（kg.d）） was better than intraperitoneal injection on breast cancer in mice（P=0.01）. The effect of endostar via micro pump combined with epirubicin was equivalent with intraperitoneal injection of two drugs（P〉0.05）, and the adverse events in former groups were reduced. Conclusion The effect of endostar via micro pump combined with epirubicin is equivalent with intraperitoneal injection of two drugs, which provides an experimental basis for the possibility of breast cancer patients carrying endostar micro pump at home, however, more clinical evidence are needed.

关 键 词：乳腺癌 重组人血管内皮抑素 表阿霉素 渗透泵 CD31 

分 类 号：R73-361[医药卫生—肿瘤] R737.9[医药卫生—临床医学]