滋阴安神方对实验性更年期模型大鼠的影响  

Effect of Ziyin Anshen Prescription on Experimental Menopause Model Rats

作　　者：彭莉[1] 苏泽琦 赵保胜 李品[1] 李佳林[1] 王停 PENG Li;SU Ze-qi;ZHAO Bao-sheng;LI Pin;LI Jia-lin;WANG Ting(Research Centre of Traditional Chinese Medicine （TCM） Constitution and Reproductive Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;Key Research Office About Famous Doctors of State Administration of TCM, Beijing 100029, China)

机构地区：[1]北京中医药大学中医体质与生殖医学研究中心,北京100029 [2]北京中医药大学北京中医药研究院,北京100029 [3]国家中医药管理局名医名方重点研究室,北京100029

出　　处：《中国实验方剂学杂志》2018年第15期137-142,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2017ZX09301011)

摘　　要：目的：观察滋阴安神方对实验性更年期模型大鼠及正常小鼠的影响,为滋阴安神方的临床应用提供实验依据。方法：采用摘除卵巢的方法建立更年期大鼠模型,随机分为更年期模型组、滋阴安神方高、中、低剂量（10,5,2.5 g·kg（-1））组、戊酸雌二醇（0.09 mg·kg（-1））组、更年安片（0.48 g·kg（-1））组,另设假手术组,每组12只。连续灌胃给药30 d,放射免疫法测定大鼠血浆促卵泡生成素（FSH）,黄体生成素（LH）,雌二醇（E2）,孕酮（P）含量,光镜下测量大鼠子宫内膜厚度。雌性ICR小鼠随机分为正常组、滋阴安神方高、中、低剂量（16.69,8.34,4.17 g·kg（-1））组和地西泮（0.001 g·kg（-1））组,每组12只。灌胃给药7 d,腹腔注射戊巴比妥钠（0.03 g·kg（-1））,记录翻正反射消失的小鼠数及入睡潜伏期。雌性ICR小鼠随机分为正常组、滋阴安神方高、中、低剂量（16.69,8.34,4.17 g·kg-1）组和百忧解（0.03 g·kg（-1））组,每组12只。灌胃给药7 d,进行悬尾实验,记录小鼠后4 min的累计不动时间。结果：与假手术组相比,模型组大鼠血浆FSH显著升高（P〈0.01）,E2明显降低（P〈0.05）,子宫内膜厚度显著下降（P〈0.01）;与模型组相比,滋阴安神方各组、戊酸雌二醇组及更年安片组大鼠血浆中E2水平及子宫内膜厚度都有一定程度升高,且滋阴安神方高、中剂量能降低血浆FSH水平。小鼠行为学实验中,滋阴安神方高剂量及地西泮可显著提高与阈下剂量戊巴比妥钠协同催眠小鼠的入睡率（P〈0.05,P〈0.01）;同时滋阴安神方低剂量及百忧解可显著缩短悬尾试验小鼠累计不动时间（P〈0.05,P〈0.01）。结论：滋阴安神方可提高更年期大鼠血浆E2水平,降低FSH水平,增加子宫内膜厚度,并具有一定的催眠及抗抑郁的作用。Objective： To observe the effect of Ziyin Anshen prescription（ZYASP） on the experimental menopause model rats and normal mice,and provide experimental basis for the clinical application of ZYASP.Method： The menopause rats models were established by ovary removal method,and were then randomly divided into the menopause model group,the ZYASP high,middle,low-dose（10,5,2. 5 g·kg（-1）） groups,the estradiol valerate 0. 09 mg·kg-1 group,and the Gengnian＇an tablet（GNAT） 0. 48 g·kg（-1） group; another sham operation group was also set; with 12 rats in each group. After intragastric administration for 30 days,plasma follicle stimulation hormone（FSH）,luteotropic hormone（LH）,estradiol（E2） and progesterone（P） in rats were measured by radioimmunoassay,and the thickness of endometrium was measured by light microscope. The female ICR mice were randomly divided into normal control group,ZYASP high,middle,low-dose（16. 69,8. 34,4. 17 g·kg（-1）） groups and diazepam 0. 001 mg ·kg（-1） group,with 12 mice in each group. After intragastric administration for 7 days,intraperitoneal injection of napental（0. 03 g·kg（-1）） was performed,and the number of mice with disappearance of righting reflex and the latency time of sleep were recorded. The female ICR mice were randomly divided into normal control group,ZYASP high,middle,low-dose（16. 69,8. 34,4. 17 g ·kg（-1））groups and the prozac（0. 03 g·kg（-1）） group,with 12 mice in each group. After intragastric administration for 7 days,the tail suspension experiment was performed,and the accumulative immobile time of the last 4 min was recorded. Result： As compared with sham operation group,the plasma FSH was significantly increased（P 0. 01）; E2 was significantly decreased（P 〈 0. 05）; and the thickness of endometrium was decreased significantly（P 〈 0. 01） in model group. As compared with the model group,E2 level in rat plasma and endometrial thickness of ZYASP groups and estradiol valerate group,and GN

关 键 词：滋阴安神方 更年期综合征 药效学 大鼠去势模型 血浆激素水平 子宫病理学 行为学 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]