小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响  

Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury

作　　者：兰瑞[1] 张勇[2] 马云枝[1] 吴涛[1] 郑海忠 王保琦 武继涛[1] LAN Rui;ZHANG Yong;MA Yun-zhi;WU Tao;ZHENG Hai-zhong;WANG Bao-qi;WU Ji-tao(The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China;The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China;Henan University of Chinese Medicine, Zhengzhou 450000, China)

机构地区：[1]河南中医药大学第一附属医院,郑州450000 [2]郑州大学第三附属医院,郑州450000 [3]河南中医药大学,郑州450000

出　　处：《中国实验方剂学杂志》2018年第15期149-154,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81503422)

摘　　要：目的：观察小续命汤对大鼠急性脑缺血再灌注损伤后脑源性神经营养因子（brain derived neurotrophic factor,BDNF）,胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor,GDNF）表达的影响。方法：60只雄性SPF级SD大鼠随机分为假手术组、模型组、小续命汤低、中、高（15,30,60 g·kg（-1））剂量组。各组大鼠于造模前3 d开始给药直至观察节点结束,采用大脑中动脉线栓法建立脑缺血再灌注损伤模型,采用苏木素-伊红（HE）染色,Nissl染色观察脑缺血再灌注损伤,采用蛋白免疫印迹法（Western blot）和免疫组织化学法检测脑缺血再灌注损伤后的BDNF,GDNF蛋白表达。结果：与模型组比较,小续命汤高、中剂量组可显著改善其神经功能缺损（P〈0.05）,减轻脑缺血再灌注引起的缺血性损伤;小续命汤高、中剂量组还可显著上调BDNF,GDNF蛋白表达（P〈0.05）。结论：小续命汤可能通过上调脑缺血再灌注后缺血半暗带区BDNF,GDNF的表达发挥脑保护的作用。Objective： To observe the effects of Xiao Xumingtang（XXMT） on brain derived neurotrophic factor（BDNF） and glial cellderived neurotrophic factor（GDNF） expression levels after acute cerebral ischemia reperfusion injury in rats. Method： A total of 60 male Sprague-Dawley rats were randomly divided into 5 groups：sham operation group（Sham）,model group（IR）,and XXMT low dose,middle dose and high dose groups（15,30,60 g·kg（-1）·d（-1））. The Drugs were given from 3 days efore modeling to the end of observation nodes. Cerebral ischemia reperfusion injury models were established by using middle cerebral artery occlusion. Then the pathological changes were estimated by hematoxylin-eosin（HE） staining and Nissl staining at 24 h after reperfusion. BDNF and GDNF expression levels after cerebral ischemia reperfusion injury were quantitatively determined by Western blot and immunohistochemistry. Result： The results indicated that XXMT middle and high dose（30,60 g·kg（-1）·d（-1）） treatment significantly improved neurologic impairment（P〈 0. 05）,reduced ischemic injury,and up-regulated the expression levels of BDNF and GDNF as compared with IR group（P〈 0. 05）.Conclusion： These findings indicated that XXMT treatment may exert the neuroprotective effect on cerebral ischemia by up-regulating expression levels of BDNF and GDNF.

关 键 词：小续命汤 急性脑缺血再灌注损伤 脑源性神经营养因子 胶质细胞源性神经营养因子 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]