生姜中姜黄素的匀浆结合有机溶剂法提取工艺优化  

Optimization of Homogenization and Organic Solvent Extraction of Curcumin in Ginger

作　　者：蔡婧婧 唐晓珍[1] 位思清 孙彩翼 王雪[1] 孙静雯 李宁阳 CAI Jing-jing;TANG Xiao-zhen;WEI Si-qing;SUN Cai-yi;WANG Xue;SUN Jing-wen;LI Ning-yang(Shandong Agricultural University, Tai＇an 271018, China;Shandong Foreign Trade Vocational College, Tai＇an 271000, China)

机构地区：[1]山东农业大学,山东泰安271018 [2]山东省外贸职业学院,山东泰安271000

出　　处：《中国调味品》2018年第7期26-32,共7页China Condiment

基　　金：山东省2016年度农业重大应用技术创新项目(2130106);山东省自然科学基金(ZR2014CM035);国家科技支撑计划子课题(2012BAD33B07);泰安市科技发展项目(20113075)

摘　　要：全球姜黄素需求量大增,其原料姜黄亩产约1000kg,而生姜亩产5000~7500kg,二者含量接近,组分相似,若能用生姜中姜黄素代替姜黄中姜黄素,经济效益十分显著。试验以优质安丘大姜为原料,利用响应面法优化生姜中姜黄素的匀浆结合有机溶剂法的提取工艺,为下步纯化提供粗提物,在单素试验基础上,以乙醇体积分数、匀浆时间、搅拌时间、料液比为自变量,以姜黄素的含量为响应量,以响应面分析法研究各自变量交互作用及其对姜黄素含量的影响,模拟得到二次多项式回归方程,进一步得到最佳提取方案为乙醇浓度93.38%,生姜与93.38%乙醇料液比1∶4(g/mL),匀浆时间3.49min,搅拌时间30.01min,此条件下姜黄素的含量为2.34mg/g,与预测值吻合,具有实用价值。Global demand for curcumin has increased significantly,its raw material yield is about 1000 kg per mu,and ginger yield is 5000~7500 kg per mu,the yield of curcumin in ginger is similar to that of curcumin in turmeric,and they have similar components,so if curcumin in ginger can be used instead of curcumin in turmeric,the economic efficiency is very significant.In this experiment,high-quality Anqiu ginger is as raw material,response surface methodology is used to optimize ginger extract by a combination of homogenization and organic solvent extraction process for providing crude extract for the next-step purification.On the basis of single-factor experiments with ethanol volume fraction,homogenization time,stirring time and solid-liquid ratio as the independent variables,the content of curcumin is as the response,and the interaction between each variable and its effect on the content of curcumin are investigated by response surface methodology.Polynomial regression equation is obtained.The best extraction scheme is ethanol concentration of 93.38%,ratio of ginger to 93.38%ethanol of 1∶4（g/mL）,homogenization time of 3.49 min and stirring time of 30.01 min.Under these conditions,the curcumin content is 2.34 mg/g,which is in line with the predicted value,it has the practical value.

关 键 词：生姜 姜黄素 提取 匀浆法 响应面分析 

分 类 号：TS201.1[轻工技术与工程—食品科学]