应用钛夹法的小鼠袖状胃切除术模型的效果研究  

Effects of sleeve gastrectomy in mice by using titanium clip

作　　者：李培鑫[1] 张忠涛[2] 黄虎 Li Peixin;Zhang Zhongtao;Huang Hu(Department of Comprehensive Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China)

机构地区：[1]首都医科大学附属北京友谊医院医疗保健中心外科,100050 [2]首都医科大学附属北京友谊医院普外科 [3]东卡罗莱纳大学人体机能学系、生理系、人类性能研究室、东卡罗莱纳糖尿病与肥胖研究所,美国北卡罗莱纳州格林威尔市27834

出　　处：《国际外科学杂志》2018年第7期460-464,F0004,共6页International Journal of Surgery

摘　　要：目的介绍一种新型的应用钛夹法的小鼠袖状胃切除（SG）模型的建立细节，探讨其是否与传统的连续缝合法的SG术模型效果一致。方法雄性FVB—Tg（NPY—hrGFP）小鼠31只，6周龄，29～31g，经高脂饮食诱导肥胖后采用随机数字表法分成SG（缝合）组8只，SG（钛夹）组15只，假手术组8只；SG（缝合）组手术采用目前文献中应用的传统连续缝合方法封闭胃断端，SG（钛夹）组采用钛夹封闭胃断端，假手术组仅分离胃周韧带；记录SG（缝合）组和SG（钛夹）组小鼠的手术时间、术后病死率，观察SG（缝合）组、SG（钛夹）组与假手术组小鼠术后的进食量及体重变化。计量资料采用均数±标准差（元x±s）表示，两组问比较采用独立样本t检验，3组间数据总体方差相等的采用方差分析（最小显著性LSD法）两两比较，各组内术前、术后数据采用配对t检验比较。结果SG（缝合）组小鼠手术时间（44．60±3．13）min，SG（钛夹）组手术时间（28．25±2．77）min，SG（钛夹）组较SG（缝合）组显著减少（P≤0．01）；SG（缝合）组小鼠病死率为37．5％，SG（钛夹）组小鼠病死率为20．0％，两组SG组小鼠之间病死率差异无统计学意义（P=0．621）。两组SG组小鼠术后平均进食量分别较假手术组显著减少（P≤0．01），而两组SG组小鼠之间术后平均进食量差异无统计学意义（P=0．149）；术后6周，SG（缝合）组小鼠体重下降（17．67±5．06）％，SG（钛夹）组小鼠体重下降（18．76±7．87）％，而假手术组小鼠体重上升（7．17±2．31）％，两组SG组体重较假手术组均显著减少（P≤0．01），两组SG组小鼠之间体重差异无统计学意义（P=0．746）。结论应用钛夹法的小鼠SG手术模型可达到与传统连续缝合法的SG手术模型一样的减少进食量和降低体重的效果；另外，由于显著减少手术时间，可�Objective To introduce details of a new procedure of sleeve gastrectomy in mice by using titanium clip, and to observe whether this model has the same post-surgical metabolic effects as traditional procedure of sleeve gastrectomy in mice by using running stitch. Methods Thirt-one male FVB-Tg （NPY-hrGFP） mice, 6 weeks （29-51 g） were randomly divided into SG （running stitch） group （n = 8）, SG （titanium clip） group （n = 15） and Sham Operation （SO） group （n = 8） by using random number table. Titanium clip was applied to close the side incision of stomachin SG （titanium clip） group, while running stitch was used in SG （running stitch） group which was detailed described in current references. Ligaments around stomach were detached without any stomach incision in SO group. Operation duration, mortality, and changes of post-surgical body weight and food intake were observed. Measured data were expressed as mean ~ standard deviation （x± s）. Independent sample t test was used for comparison between the two groups. The variance of the data between the three groups was equal and analysis of variance （least significant LSD method） was used to compare each group. Preoperative and postoperative data were compared using a paired t test. Results Operation duration of SG （ running stitch） group and SG （ titanium clip） group were （44.60 ± 3.13） min and （28.25 ± 2.77） min, respectively. Compared with SG （running stitch） group, operation duration was significantly reduced in SG （titanium clip） group （P≤ 0.01 ）. Mortality of SG （running stitch） group and SG （titanium clip） group were 37.5% and 20%, respectively, which has no significant difference （P = 0. 621 ）. Compared with SO group, postoperative food intake was significantly reduced in both two SG groups （P≤ 0.01 ）, but there＇s no difference between two SG groups （P =0. 149）. At the end of the 6-week-postoperative, body weights of SG （running stitch） group and SG （titaniu

关 键 词：小鼠 肥胖症 减肥手术 胃切除术 钛夹法 

分 类 号：R-332[医药卫生]