miRNA-455-3p对HCT116结肠癌细胞迁移、侵袭的影响及机制研究  

Effect of miR-455-3p overexpression on migration and invasion of colon cancer HCT116 cells and possible mechanism

作　　者：刘益娟 张琳[2] 郑建涛[2] 李良庆[2] LIU Yijuan;ZHENG Jiantao;LI Liangqing(Department of Gastroenterology;Department of Gastrointestinal Surgery,First Affiliated Hospital of Fujian Medical University,Fuzhou,Fujian Province,350005,China)

机构地区：[1]福建医科大学附属第一医院消化内科,福州350000 [2]福建医科大学附属第一医院胃肠外科,福州350000

出　　处：《第三军医大学学报》2018年第14期1286-1290,共5页Journal of Third Military Medical University

基　　金：福建省医学创新课题项目(2017-CX-29)~~

摘　　要：目的探讨miRNA-455-3p对HCT116结肠癌细胞迁移、侵袭的影响及机制研究。方法采用脂质体LipofectamineTM3000将miR-455-3p mimics和miR-455-3p NC转入HCT116结肠癌细胞中,Real-time PCR检测细胞中miR-455-3p表达,MTT法检测细胞活力,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力,Western blot检测细胞中基质金属蛋白酶2(matrix metalloproteinases2,MMP2)、MMP9、磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI3K)/蛋白激酶B(protein kinase B,AKT)信号通路相关蛋白的表达。结果与miR-455-3p NC组比较,miR-455-3p mimics组miR-455-3p表达量显著提高(P<0.01),细胞活力、迁移能力、侵袭能力均显著降低(P<0.01),MMP2、MMP9、PI3K及p-AKT表达量均显著下调(P<0.01)。结论 miR-455-3p mimics可能通过阻断PI3K/AKT信号通路抑制HCT116细胞的迁移及侵袭。Objective To investigate the effect of miR-455-3p overexpression on the migration and invasion of colon cancer HCT116 cells and explore the underlying mechanism. Methods HCT116 cells were transfected with miR-455-3p mimics or miR-455-3p NC using LipofectamineTM 3000, and the expression of miR-455-3p in the cells was detected with real-time PCR. The changes in the viability of the transfected cells was assessed using MTT assay, and the alterations in the cell migration and invasion abilities of the cells were investigated using scratch wound healing assay and Transwell assay, respectively. The expression levels of matrix metalloproteinases 2 （MMP2）, MMP9, and the proteins related with the phosphatidylinositol 3 kinase （PI3K）/protein kinase B （AKT） signaling pathway were detected using Western blotting. Results Compared with miR-455-3p NC, transfection with miR-455-3p mimics resulted in significantly up-regulated expression of miR-455-3p （P〈0.01）, obviously lowered cell viability and migration and invasion abilities （P〈001）, and significant down-regulation of the expression of MMP2, MMP9, PI3K and p-AKT （P〈0.01） in HCT116 cells. Conclusion Overexpression of miR-455-3p can inhibit the migration and invasion of HCT116 cells in vitro possibly by blocking the PI3K/AKT signaling pathway.

关 键 词：miR-455-3p HCT116结肠癌细胞 迁移 侵袭 

分 类 号：R394.3[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]