外周血T细胞亚群计数与恶性肿瘤的相关性研究  

Study on the correlation between T-lymphocyte subgroup count of peripheral blood and malignant tumor

作　　者：陈娟[1] 毕明宏[1] 赵论[1] 周海荣[1] CHEN Juan;BI Ming-hong;ZHAO Lun;ZHOU Hai-rong(Department of Medical Oncology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,Chin)

机构地区：[1]蚌埠医学院第一附属医院肿瘤内科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2018年第5期592-595,共4页Journal of Bengbu Medical College

摘　　要：目的:探讨外周血T细胞亚群计数在恶性肿瘤中临床意义。方法:采用流式细胞术检测80例恶性肿瘤病人和100名健康体检者外周血CD3^+、CD4^+和CD8^+细胞计数以及CD4^+/CD8^+比值。结果:恶性肿瘤病人CD3^+、CD4^+、CD8^+细胞计数及CD4^+/CD8^+比值均较健康人群显著降低(P<0.01)。亚组分析结果显示,各恶性肿瘤组的CD3^+和CD4^+细胞计数均较对照组下降(P<0.05~P<0.01),CD8^+细胞计数与对照组比较差异均无统计学意义(P>0.05),仅肺癌组CD4^+/CD8^+比值低于对照组(P<0.05);首诊组和进展组恶性肿瘤病人的所有观察指标差异均无统计学意义(P>0.05);男性恶性肿瘤病人的CD4^+细胞计数低于女性病人(P<0.05)。结论:恶性肿瘤病人的CD3^+、CD4^+、CD8^+细胞水平以及CD4^+/CD8^+比值存在一定程度的失衡,通过检测这些指标,可以了解病人机体的免疫状态,直接或者间接为肿瘤的免疫治疗提供依据。Objective： To investigate the clinical significance of T-lymphocyte subgroup count of peripheral blood in malignant tumor.Methods： The amount of CD3^＋cells,CD4^＋cells,CD8^＋cells and ratio of CD4^＋/CD8^＋of peripheral blood in 80 malignant tumor patients and 100 healthy people were determined by flow cytometry. Results： Compared with healthy people,the amount of CD3^＋cells,CD4^＋cells,CD8^＋cells and ratio of CD4^＋/CD8^＋in patients with malignant tumor were significantly decreased（ P〈0. 01）. The results of subgroup analysis showed that the counts of CD3^＋cells and CD4^＋cells in malignant tumor patients decreased compared with control group（ P〈0. 05 to P〈0. 01）,the difference of the count of CD8^＋cells between two groups was not statistically significant（ P〈0. 05）,and ratio of CD4^＋/CD8^＋in lung cancer group was lower than that in control group（ P〈0. 05）. The difference of the parameter of malignant tumor patients between the first diagnosis group and progression group was not statistically significant（ P〈0. 05）. The amount of CD4^＋cells in amale patients was lower than that in female patients（ P〈0. 05）. Conclusions： The imbalance of the counts of CD3^＋cells,CD4^＋cells,CD8^＋cells and ratio of CD4^＋/CD8^＋in malignant tumor patients are identified,which can be used to evaluate the immunity status of tumor patients,and provide a scientific evidence in immunotherapy of tumor.

关 键 词：肿瘤 恶性 T细胞 流式细胞术 

分 类 号：R730.3[医药卫生—肿瘤]