慢性应激对大鼠牙周组织IL-1β和TNF-α表达的影响  

The effect of chronic stress on IL-1β and TNF-α expression in periodontal tissues of rats

作　　者：宋红丽[1] 李斌[1] 陈耀忠[1] Song Hongli;Li Bin;Chen Yaozhong(Department of Stomatology , Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing 210009, China)

机构地区：[1]东南大学附属中大医院口腔科,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2018年第6期843-847,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市卫计委项目(YKK15245)

摘　　要：目的:探讨慢性应激促进牙周炎的可能机制。方法:选择慢性不可预知应激大鼠模型(chronic unpredictable stress,CUS)。将大鼠分为正常对照和慢性应激组(4周后根据糖水消耗实验将应激组又分为抑郁组和非抑郁组)。每周记录大鼠体重,8周后称取肾上腺重量。分别使用糖水消耗实验、酶联免疫法、蛋白质印迹法(Western blot),实时荧光定量PCR(Real-time PCR)检测各组大鼠抑郁样行为,血浆皮质醇和胰岛素水平,牙周组织总的及磷酸化胰岛素受体水平及白细胞介素1β(interleukin-1β,IL-1β)和肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)含量。结果:慢性应激4周后,约59%的大鼠表现出明显的快感缺失;与对照组相比,无论是否存在快感缺失,应激3周后所有大鼠均出现体重下降。与对照组相比,应激8周后大鼠肾上腺重量明显增加、血浆皮质醇水平明显升高;应激大鼠血浆胰岛素水平下降,牙周组织内磷酸化的胰岛素受体底物1表达下降;应激大鼠牙周组织内IL-1β和TNF-α的m RNA表达增加,上述指标在应激组内(抑郁组和非抑郁组之间)无显著性变化。结论:应激本身,而非应激引起的情绪障碍,引起了下丘脑-垂体-肾上腺(HPA)轴紊乱,从而导致胰岛素信号通路异常,牙周组织炎性因子表达明显增加。这可能是慢性应激后易患牙周炎的可能原因。Objective：To explore the mechanism of the chronic stress contribution to periodontitis. Method：Sprague-Dawley rats（2 months） were received chronic unpredicted mild stress （CUS）for eight weeks. Then, four weeks later, they were devided into susceptible （depressive） or unsusceptible （resistant） groups according to sucrose preference test. Total body and adrenal weights of rats were weighed weekly and at 8 week, respectively. The levels of plasma corticosterone and insulin, the total insulin receptor（IRS）and activity -dependent phosphorylated IRS in the periodontal tissues, and the mRNA expression of IL-1β and TNF-α in the periodontal tissues were detected by enzyme-linked immunosorbent assay （ELISA）, Western blot and Real-time PCR, respectively. Results：After 4 weeks of CUS, 59% of rats showed a decrease of sucrose preference indicating anhedonic or depressive-like behavior （CMSA）. Compared with controls, all the stressed rats showed decreased weight after three weeks ; after eight weeks, they also exhibted increased corticosterone and decreased insulin in plasma, decreased p-IRS and increased mRNA levels of IL-1β and TNF-α in the periodontal tissue. The depressive andresitant groups showed no significant differences in above measures. Conclusion ： The stress itself, rather than stress- induced mood disorders, leads to HPA axis disorders, abnormality of insulin signal pathway, and increased expression of periodontal tissue inflammatory factor. These changes may serve as the mechanism in contibuting to stress susceptible periodontal disease.

关 键 词：慢性应激 HPA轴 胰岛素信号通路 炎症因子 

分 类 号：R782.42[医药卫生—口腔医学]