转化转录因子HaNAC1基因提高马铃薯的抗逆性  

Stress Resistance of Potato Enhanced by Transcription Factor HaNAC1 Gene

作　　者：张丽[1] 程永芳[2] 巩檑[1] 甘晓燕[1] 聂峰杰[1] 陈虞超[1] 石磊[1] 宋玉霞[1] Zhang Li;Cheng Yongfang;Gong Lei;Gan Xiaoyan;Nie Fengjie;Chen Yuchao;Shi Lei;Song Yuxia(Agricultural Bio-Technology Centre, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002;College of Life Science in Ningxia University, Yinchuan, 750003)

机构地区：[1]宁夏农林科学院农业生物技术研究中心,银川750002 [2]宁夏大学生命科学学院,银川750003

出　　处：《分子植物育种》2018年第14期4623-4631,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31560053);宁夏农业育种专项(2014NYYZ01);宁夏自然科学基金(NZ17124;NZ16123);宁夏农林科学院科技创新先导资金项目(NKYJ-17-19;NKYJ-17-07;NKYJ-17-21;NKYJ-16-12;DWHZC-2017002)共同资助

摘　　要：为提高马铃薯抗逆性,利用农杆菌介导法,将携带目的基因HaNAC1质粒分别导入马铃薯栽培种‘大西洋’、‘费乌瑞它’和‘青薯168’中。对卡那霉素(Kan)抗性转化株系经PCR扩增检测,共得到63个阳性株系。利用Southern blot对随机选取的7个转基因阳性株系进行检测,结果表明HaNAC1均已整合到各转基因株系中。对63个阳性株系进行RT-PCR检测,共有55个株系中出现目的基因条带,说明HaNAC1在转录水平有表达。以90 mmol/L NaCl和15%PEG分别添加至培养基中模拟盐和旱胁迫处理,从相对株高、相对根长等形态指标对32个‘大西洋’转基因株系进行筛选获得了4个优系,丙二醛、脯氨酸等生理指标也进一步证实HaNAC1基因转化可有效提高转基因马铃薯的抗逆性。In order to enhanced the stress tolerance of potato,the plasmid encoding HaNAC1 gene was transferred into cultivars of Atlantic,Favorita and Qingshu 168 respectively via Agrobacterium tumefaciens mediated method.After PCR amplification detection,63 positive transgenic lines were obtained from Kanamycin resistant lines.7 positive lines were chosen randomly for Southern blot analysis,the results of which confirmed that HaNAC1 gene had been integrated into the potato.The RT-PCR analysis of 63 positive lines showed that 55 lines had positive target bands,indicating that HaNAC1 expressed at the transcription level.90 mmol/L NaCl and 15% PEG were added to the culture medium to simulate salt and drought stress,and 4 best lines were screened from 32 Atlantic transgenic lines according to relative plant height and relative root length.Malondialdehyde,proline and other physiological indicators further confirmed that HaNAC1 gene transformation could effectively improve the resistance of transgenic potato.

关 键 词：马铃薯 根癌农杆菌 HaNAC1基因 抗逆性 

分 类 号：S532[农业科学—作物学]