丙二醛、超氧化物歧化酶及内毒素在大鼠布加综合征模型肝损伤中的表达及意义  

Expression and significance of malonaldehyde, superoxide dismutase and endotoxin in liver injury model of Budd-Chiari syndrome in rats

作　　者：朱楠 成德雷 方伟伟 吕维富 Zhu Nan;Cheng Delei;Fang Weiwei;Lyu Weifu(Department of Radiology,People＇s Hospital of Wuhe County Bengbu City,Wuhe 233300,Chin)

机构地区：[1]安徽省蚌埠市五河县人民医院影像科,五河233300 [2]中国科学技术大学附属第一医院介入放射科

出　　处：《中华放射学杂志》2018年第7期554-559,共6页Chinese Journal of Radiology

基　　金：安徽省自然科学基金项目（1708085QH218）

摘　　要：目的探讨丙二醛（MDA）、超氧化物歧化酶（SOD）及内毒素（ET）在大鼠布加综合征（BCS）肝损伤模型中的表达及意义。 方法通过部分结扎大鼠肝后段下腔静脉建立BCS动物模型。所有实验大鼠分为3组：健康对照组（12只）、模型组（48只）和假手术组（48只），模型组及假手术组又各分4个亚组（实验1、3、6、12周），每亚组12只大鼠。行DSA证实造模成功后每组随机抽取9只大鼠处死，留取血清及肝脏组织，采用双抗体夹心法检测肝匀浆及血清中MDA、SOD及ET的表达量。通过Levene法检验所测数据符合方差齐性后，以方差分析比较组内、组间的差异，采用LSD法行组内、组间两两比较，采用Pearson法行MDA、SOD及ET相关性分析。 结果模型组大鼠术后肝匀浆及血清中各时间点的MDA、SOD、ET值与对照组及假手术组中相应指标比较，差异均具有统计学意义（MDA：肝匀浆F值分别为52.906、219.016，血清分别为21.573、43.878；SOD：肝匀浆F值分别为22.927、19.317，血清分别为10.841、31.643；ET：肝匀浆F值分别为33.588、105.515，血清分别为40.832、46.323；P值均〈0.05）。模型组术后各时间点肝匀浆MDA值差异有统计学意义（F=7.716，P=0.001），血清MDA值差异无统计学意义（F=1.965，P=0.139）；模型组肝匀浆及血清SOD及ET值在术后各时间点比较，差异均有统计学意义（SOD：F值分别为17.053、7.903；ET：F值分别为19.870、39.372；P值均〈0.05），两者在肝匀浆及血清中含量均从术后1周开始逐渐上升，6周达峰值，第12周稍下降。模型组各时间点肝匀浆MDA及ET较假手术组上升值均高于血清中相应上升值。模型组肝匀浆及血清中MDA与SOD均为负相关（r分别为－0.814、－0.591，P均为0.001）、与ET均为正相关（r分别为0.761、0.422，P分别为0.004、0.001），SOD与ET均为负相关（r分别为－0.726、－0.490，P均为0.001）�ObjectiveTo investigate the expression and significance of malonaldehyde （MDA）, superoxide dismutase （SOD） and endotoxin （ET） in liver injury model of Budd-Chiari syndrome （BCS） in rats. MethodsThe animal model of BCS was established by partially ligating the inferior vena cava of the posterior segment of liver in rats. The experimental animals were divided into three groups： control group （12 rats）, model group （48 rats） and sham operation group （48 rats）. The model group and sham operation group were divided into four subgroups （1, 3, 6, 12 weeks） of 12 rats each. After the success of modeling, being confirmed by digital subtraction angiography （DSA）, nine rats in each group were sacrificed at random respectively, where their serums and liver tissues was collected. The levels of MDA, SOD and ET in both liver homogenate and serum were examined respectively. ANOVA was used to compare the total difference between groups and within group of each measurement data. The LSD method was used to do multiple comparison within group and between groups. Pearson method was used to do correlation analysis of hypoxia markers. ResultsThe levels of MDA, SOD and ET in liver homogenate and serum at different time points in model group were significantly different from those in control group and sham operation group （MDA： liver homogenate （F=52.906, 219.016）, serum （F=21.573, 43.878）;SOD： liver homogenate （F=22.927, 19.317）, serum （F=10.841, 31.643）;ET： liver homogenate （F= 33.588, 105.515）, serum （F= 40.832, 46.323）;P〈0.05）. The total difference of the MDA level in serum at each time point after the operation was not statistically significant in model group（F=1.965, P=0.139）, but that of liver homogenate in the model group was statistically significant （F=7.716, P=0.001）. The SOD and ET levels in both liver homogenate and serum of model group were compared within groups at different time points after operation respectively, and the overall diff

关 键 词：缺氧 内毒素类 氧自由基 布加综合征 

分 类 号：R-332[医药卫生] R575