BCA法优化补骨脂蛋白质的提取工艺  

Optimization of the Extraction Technology of Protein from Psoralea Corylifolia by BCA Method

作　　者：孙丽丽[1] 麦雨婷 谷婷婷 孙道裕 刘艳萍 戴春燕[3] 刘红[1] SUN Lili;MAI Yuting;GU Tingting;SUN Daoyu;LIU Yanping;DAI Chunyan;LIU Hong(School of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;Department of Medical Equipment,Haikou People’s Hospital,Haikou 570208,China;Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education,Haikou 571158,China)

机构地区：[1]海南师范大学化学与化工学院,海南海口571158 [2]海口市人民医院医疗设备处,海南海口570208 [3]热带药用植物化学教育部重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2018年第2期158-163,共6页Journal of Hainan Normal University（Natural Science）

基　　金：国家自然科学基金地区项目(31660097);海南师范大学大学生创新创业训练计划项目(cxcyxj2016012)

摘　　要：采用碱提酸沉法提取补骨脂蛋白质,以二喹啉甲酸(BCA)法测定的蛋白质溶出率为指标,通过单因素实验和正交实验优化补骨脂蛋白质的碱提工艺条件,考察浸提p H、料液比、温度、时间等因素对补骨脂蛋白质提取的影响.结果表明,四种提取因素的影响从大到小的顺序为:浸提p H>料液比>温度>时间.最佳工艺条件为浸提p H 10.0、料液比(g/m L)1∶20、温度45℃、浸提时间150 min,此条件下补骨脂蛋白质溶出率为23.69%.以BCA法测定酸沉蛋白质浓度,确定补骨脂蛋白质的最大沉淀量p H为4.5,蛋白质的最大沉淀率为55.16%.对比发现理论提取率与实际提取率基本相同,证明BCA法测定补骨脂蛋白质的方法准确、可靠,为补骨质蛋白质的进一步研究利用奠定了基础.The extraction rate which measured by BCA method was applied to evaluate the protein extraction from Psoralea corylifolia by alkali extraction and acid precipitation method. Both of single factor experiment and orthogonal test were used to optimize the four parameters of alkali extraction technology. Results show that the most important factor is the extraction PH, then is the ratio of solid to the liquid, the extraction temperature, and the extraction time. The most suitable extraction conditions for Psoralea corylifolia protein were as follows： suitable extraction pH is 10.0; ratio of material to the liquid is 1∶ 20 ; extraction temperature is 45℃ and the extraction time is 150 min. Under the determined extraction conditions, protein dissolving rate is 23.69%. As the same, the acid precipitation rate under different pH obtained by BCA method was compared, and the best precipitation rate pH is 4.5, and the largest precipitation rate is 55.16%. In the end, by comparing theoretical protein extraction rate with the practical protein extraction rate, the research found that BCA method was creditable.

关 键 词：补骨脂 蛋白质 碱提酸沉法 二喹啉甲酸(BCA)法 正交实验 

分 类 号：O157.5[理学—数学]