枸杞子、淫羊藿的提取物与海参、牡蛎肉的冻干粉合用缓解体力疲劳作用研究  

Study on anti-fatigue effects of composite extraction of Herba epimedii,Medlar,sea cucumber and Oyster in mice

作　　者：陈垚[1] 吕志敏[1] 殷方美 高景芳[1] 孙川喻[1] C HEN Yao;LV Zhi-min;YIN Fang-mei;GAO J ing-fang;SUN Chuan-yu(Rizhao City People＇s Hospital,Rizhao,Shandong,276800,Chin)

机构地区：[1]日照市人民医院,山东日照276800

出　　处：《预防医学论坛》2018年第4期316-319,共4页Preventive Medicine Tribune

摘　　要：目的研究枸杞子、淫羊藿的提取物与海参、牡蛎肉的冻干粉合用缓解体力疲劳的作用效果。方法按《保健食品检验与评价技术规范》缓解体力疲劳检测评价方法,将192只ICR(institute of cancer research)雄性小鼠随机分为阴性溶剂对照组、低(0.14g/kg·bw)、中(0.27g/kg·bw)、高剂量(0.80g/kg·bw)组,每组48只,连续灌胃给药30d后,测定小鼠负重游泳时间、游泳后血清尿素、血乳酸含量和肝糖原含量。结果中、高剂量组小鼠负重游泳时间分别为(549±97)s、(541±91)s,较对照组(408±75)s明显增加(P<0.01);中、高剂量组小鼠血清尿素分别为(12.43±0.69)mmol/L、(12.53±0.85)mmol/L,较对照组(13.49±0.79)mmol/L明显降低(P<0.01);低、中、高剂量组肝糖原分别为(5.61±0.55)、(6.33±0.71)、(6.38±0.75)g/100g,均高于对照组(P<0.01)。中剂量组小鼠运动血乳酸含量显著低于对照组(P<0.01)。结论枸杞子、淫羊藿的提取物与海参、牡蛎肉的冻干粉合用具有缓解体力疲劳的作用。Objective To evaluate the alleviating physical fatigue function of soft capsule of Herba epimedii,Medlar,sea cucumber and Oyster in mice. Methods In accordance with method for the assessment of alleviating physical fatigue function in Technical Standards for Testing Assessment of Health Food.A total of 192 male mice were randomly divided into 4 groups：the negative control group,and composite extraction at different doses of low（0.30 g/kg）,medium（0.60 g/kg）,and high（1.80 g/kg）dosage groups,with 48 mice in each group.The extractions were consistently administered intragastricly for 30 days.Subsequently,indicators including the loaded swimming time,lactic acid content in the whole blood,urea nitrogen in the serum and hepatic glycogen content were measured. Results Compared with the control group,the loaded swimming time（549±97,541±91）s and of the mice in medium and high dose groups was significantly prolonged（P〈0.01）,but serum urea level（12.43±0.69,12.53±0.85）mmol/L decreased significantly（P〈0.01）.The hepatic glycogen content of each dose groups was significantly increased（P〈0.01）.The blood lactic acid content of the mice after swimming medium dose groups was significantly decreased（P〈0.01）.No statistically significant differences were found in body weight between each dose group and the negative control group（all P〈0.05）. Conclusion The composite extraction of Herba epimedii,Medlar,sea cucumber and Oyster has the function of alleviating physical fatigue.

关 键 词：枸杞子 淫羊藿 海参 牡蛎 抗疲劳 小鼠 

分 类 号：R96[医药卫生—药理学]