傅立叶变换红外光谱技术对阪崎肠杆菌及奇异变形杆菌的分类鉴定  

Discrimination and Identification of Enterobacter sakazakii and Proteus mirabilis by Fourier Transform-infrared Spectroscopy

作　　者：张占林[1] 乔勇升[1] 王萍[1] ZHANG Zhan-lin;QIAO Yong-sheng;WANG Ping(Taizhou Institute for Food and Drug Control,Taizhou 225300,Jiangsu,Chin)

机构地区：[1]泰州市食品药品检验所,江苏泰州225300

出　　处：《食品研究与开发》2018年第12期150-154,共5页Food Research and Development

摘　　要：运用傅立叶变换红外光谱技术(Fourier transform infrared spectroscopy,FT-IR)对2株不同来源的阪崎肠杆菌及奇异变形杆菌建立数据库并进行快速分类鉴定。利用FT-IR技术采集菌体生化构成光谱信息,对数据预处理后借助计算机和化学计量学方法进行主成分分析(principal component analysis,PCA)和系统聚类分析(hierarchical cluster analysis,HCA)。结果显示,PCA和HCA两种分析方法成功将阪崎肠杆菌和奇异变形杆菌进行区分,且不同来源的2株阪崎杆菌也得到明显区分。FT-IR技术结合计算机与化学计量学方法,对阪崎肠杆菌及奇异变形杆菌的分类鉴定方面提供了一种有效的工具。Fourier transform-infrared spectroscopy（FT-IR）was used to establish a rapid method of discrimination and identification of the two Enterobacter sakazakii from different sources and Proteus mirabilis. Spectra information of the biochemical composition of three strains was collected by FT-IR and analyzed using computer and chemometric methods for principal component analysis（PCA） and hierarchical cluster analysis（HCA）after preprocessing. The results showed that Enterobacter sakazakii and Proteus mirabilis were differentiated successfully by the two methods of PCA and HCA,and the two Enterobacter sakazakii from different sources got a clear distinction. FT-IR combined with chemometric methods gave a credible tool for discrimination and identification of Enterobacter sakazakii and Proteus mirabilis.

关 键 词：傅立叶变换红外光谱 阪崎肠杆菌 奇异变形杆菌 分类鉴定 主成分分析 系统聚类分析 

分 类 号：O657.33[理学—分析化学] TS207.4[理学—化学]