CD44基因多态性对原发性肝癌细胞局部T细胞亚群浸润的影响  

Effect of CD44 gene polymorphism on infiltration of local T cell subsets in primary liver cancer tissue

作　　者：苗凤华[1] 陈晓[1] 石磊[1] 马筱慧[1] Feng-hua Miao;Xiao Chen;Lei Shi;Xiao-hui Ma(Cancer Center,the First Hospital of Jilin University,Changchun,Jilin 130021,China)

机构地区：[1]吉林大学第一医院肿瘤中心,吉林长春130021

出　　处：《中国现代医学杂志》2018年第17期69-72,共4页China Journal of Modern Medicine

基　　金：吉林省卫生计生委资助项目(No:2016J053)

摘　　要：目的探讨CD44基因多态性对原发性肝癌细胞局部T细胞亚群浸润的影响。方法前瞻性收集2017年1月-2017年12月吉林大学第一医院收治的原发性肝癌患者109例,根据CD44基因rs4756195位点单核苷酸多态性,将患者分为AA组、AG组和GG组,观察3组患者局部组织T细胞亚群浸润情况。采用流式细胞术检测CD4^+T、CD8^+T、Treg及Th17细胞,免疫组织化学法检测白介素-2(IL-2)、IL-10肿瘤生长因子-β(TGF-β)和IL-17的阳性表达,聚合酶链反应检测CD44基因rs4756195位点单核苷酸多态性。结果 AA组、AG组、GG组CD4^+T细胞分别为(21.08±2.12)%、(19.43±1.98)%、(19.12±2.09)%,差异有统计学意义(P<0.05);CD8^+T细胞分别为(28.83±2.52)%、(25.52±2.71)%、(24.91±2.29)%,差异有统计学意义(P<0.05);调节性T细胞分别为(6.12±1.47)%、(8.12±1.71)%、(8.24±1.92)%,差异有统计学意义(P<0.05);IL-2阳性表达率分别为82.09%、47.83%、68.42%,差异有统计学意义(P<0.05);IL-10阳性表达率分别为46.27%、69.57%、73.68%,差异有统计学意义(P<0.05);TGF-β阳性表达率分别为41.79%、77.27%、78.95%,差异有统计学意义(P<0.05)。结论原发性肝癌患者CD44基因rs4756195位点呈多态性分布,与肝癌组织中的免疫抑制有关。Objective To investigate the effect of CD44 gene polymorphism on the local infiltration of T cell subsets in primary liver cancer tissue. Methods From January to December 2017, 109 cases of primary liver cancer patients were prospectively collected in our hospital. According to the CD44 gene rs4756195 single nucleotide polymorphism（SNP）, the patients were divided into group AA, group AG and group GG. The local tissue infiltration of T cell subsets of the three groups was studied. Flow cytometry was used to detect CD4~＋ and CD8~＋ T cells, Treg cells and Th17 cells. Immunohistochemical method was used to study the expressions of IL-2, IL-10, IL-17 and TGF-β. PCR was used to determine SNP of CD44 gene rs4756195. Results In the group AA, the group AG and the group GG, CD4~＋ T cells accounted for（21.08 ± 2.12）%,（19.43 ± 1.98）% and（19.12 ± 2.09）% respectively, there was significant difference（P〈0.05）; CD8~＋ T cells accounted for（28.83 ± 2.52）%,（25.52 ± 2.71）% and（24.91 ± 2.29）% respectively, there was significant difference（P〈0.05）; regulatory T cell occupied（6.12 ± 1.47）%,（8.12 ± 1.71）% and（8.24 ± 1.92）% respectively, the difference was statistically significant（P〈0.05）; the positive expression rate of IL-2 was 82.09%, 47.83% and 68.42% respectively, the difference was statistically significant（P〈0.05）; the positive expression rate of IL-10 was 46.27%, 69.57% and 73.68% respectively, the difference was statistically significant（P〈0.05）; the positive expression rate of TGF-β was 41.79%, 77.27% and 78.95% respectively, the difference was statistically significant（P〈0.05）. Conclusions The polymorphic distribution of CD44 gene rs4756195 locus in the patients with primary liver cancer is related to the immunosuppression in the liver cancer tissue.

关 键 词：CD44基因 原发性肝癌 T细胞亚群 多态性 

分 类 号：R735.7[医药卫生—肿瘤]