不同植物修复锰矿渣模式中根际菌群的特性及多样性研究  

Analysis of characteristics and diversity of rhizobacteria in tested phytoremediation models

作　　者：张爽 冯冲凌 欧阳林男 李刘刚 吴晓芙 ZHANG Shuang;FENG Chongling;OUYANG Linnan;LI Liugang;WU Xiaofu(College of Environmental Science and Engineering,Central South University Of Forestry and Technology,Changsha 410004,Hunan,China)

机构地区：[1]中南林业科技大学环境科学与工程学院,湖南长沙410004

出　　处：《中南林业科技大学学报》2018年第8期89-96,共8页Journal of Central South University of Forestry & Technology

基　　金：国家"十二五"科技支撑计划项目(2012BAC09B03-4);国家"十二五"科技惠民计划项目(2012GS430203);湖南省环境科学与工程重点学科建设项目

摘　　要：以锰矿渣为基质开展了植物修复中试试验,测定对比了有机菌肥处理下不同植物群落模式配置中基质重金属浓度和其他理化性质的差异,同时利用Illumina Miseq平台对各处理基质样本中菌群的16S rRNA基因V4区域进行测序,分析了细菌群落结构及多样性的变化。试验结果表明施用有机菌肥能显著降低了土壤重金属活度,改善了根际微生态环境。基因测序分析中共检测到了150 703个有效序列,基质样本中的微生物隶属34个门类和81个纲类,变形菌为优势菌群。有机菌肥的应用显著改变了基质中微生物的组成、结构与相对丰度,丰富了菌群多样性。总体上,有机菌肥对细菌群落结构的影响要大于植物群落模式的影响。RDA分析显示,决定基质细菌分布的关键环境因子是其Mn离子含量。Pilot scale phytoremediation tests using Mn mining wastes as substrate were carried out to compare the effects of bacteria containing-organic manure application and plant community model on soil heavy metal concentration and other related properties. Illumina sequencing technique was applied to analyze the characteristics of rhizobacteria and related changes in bacterial community structure and diversity. The result showed that the application of organic manure significantly reduced soil heavy metal activity and thus improved the rhizosphere＇s micro-environment. A total of 150,703 valid sequences were detected in collected substrate samples. Sequence analyses at phylum and class taxonomic levels illustrated that there were 34 identified phyla and 81 classes of which Proteobacteria was found to be the dominant bacteria. In general, the organic fertilizer application had caused significant changes in composition, structure and species relative abundance of the rhizospheric bacterial community and enriched the diversity of flora species. The impact of organic manure application on bacterial community structure was shown to be is stronger than that of plant community. RDA analysis showed that the key factor affecting the distribution of bacterial species was Mn content.

关 键 词：根际菌群 有机菌肥 中试试验 16S RRNA 锰矿 

分 类 号：S731.6[农业科学—林学] S719