沉默SLP-2可抑制人宫颈癌细胞的迁移及侵袭能力  

Silencing of SLP-2 inhibits the migration and invasion of cervical cancer cells in vitro

作　　者：张佳露 胡国林 刘磊 陈伙娣 李丹娟[1] 梁卫江[1] ZHANG Jialu;Hid Guolin;LIU Lei;CHEN Huodi;LI Danjuan;LIANG Weijiang(Department of Oncology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Oncology,Qingyuan People＇s Hospital,Qingyuan 511500,China)

机构地区：[1]南方医科大学南方医院肿瘤内科,广东广州510515 [2]清远市人民医院肿瘤内科,广东清远511500

出　　处：《南方医科大学学报》2018年第7期812-817,共6页Journal of Southern Medical University

基　　金：广东省自然科学基金(2016A030313524);南方医院院长基金(2015B009)

摘　　要：目的研究沉默SLP-2对人宫颈癌细胞迁移及侵袭能力的影响及其作用机制。方法用siRNA转染人宫颈腺癌Hela细胞及宫颈鳞癌Siha细胞作为沉默组,同时设立空白对照组及阴性对照组,转染48 h后,用Western blot检测SLP-2蛋白的表达量,用划痕实验、Transwell实验检测沉默SLP-2对Hela和Siha细胞迁移能力的影响,用基质胶Transwell实验检测沉默SLP-2对Hela和Siha细胞侵袭能力的影响,用Western blot检测沉默SLP-2后Hela和Siha细胞上皮间质转化标志分子E-cadherin、β-catenin、vimentin及上皮间质转化上游转录因子Twist蛋白表达量的变化。结果与对照组相比,Western blot结果显示沉默组SLP-2蛋白的表达量明显下降;划痕实验显示沉默组的划痕面积愈合率明显下降(P<0.01);Transwell实验及基质胶Transwell实验均显示沉默组穿过小室膜至下室的细胞数明显减少(P<0.01);Western blot结果显示沉默组上皮细胞标志分子E-cadherin及β-catenin表达升高,而间质细胞标志分子vimentin表达下降,表明Hela细胞和Siha细胞的上皮间质转化受到抑制;Western blot结果显示沉默组Twist蛋白的表达量也明显下调。结论沉默SLP-2可抑制人宫颈癌细胞上皮间质转化(EMT)的发生,使宫颈癌细胞的迁移及侵袭能力下降,其作用机制可能与下调Twist蛋白的表达有关。Objective To investigate the effect of SLP-2 silencing on the migration and invasion of human cervical cancer cells and explore the mechanism. Methods Small interfering RNA（siRNA） was used to knockdown the expression of SLP-2 in Hela cells and Siha cells. At 48 h after the transfection, the cells were examined for SLP-2 expression with Western blotting, and wound healing assay and Transwell assay were used to evaluate the changes in the cell migration; Matrigel Transwell assay was used to evaluate the changes in the invasion ability of the cells. The expressions of E-cadherin, β-catenin, vimentin and Twist in Hela and Siha cells following the transfection were detected with Western blotting. Results Compared with the control cells, siRNA transfection significantly lowered the expression of SLP-2 and suppressed the migration and invasion ability of Hela cells and Siha cells（P〈0.01）. Silencing SLP-2 induced obvious up-regulation of epithelial cell phenotype proteins E-cadherin and β-catenin, down-regulated the expression of interstitial cell phenotype protein vimentin, and lowered the expression of Twist in the cells. Conclusion Silencing SLP-2 via siRNA transfection can inhibit epithelial-mesenchymal transition of human cervical cancer cells and lower their migration and invasion abilities possibly in relation with downregulated expression of Twist.

关 键 词：宫颈癌 迁移 侵袭 SLP-2 上皮间质转化 

分 类 号：R737.33[医药卫生—肿瘤]