miR-128对胶质母细胞瘤U87细胞侵袭性的影响及作用机制  

Effects of Mir-128 on Invasion of Glioblastoma U87 Cells and Its Mechanism of Action

作　　者：常亮[1] 王鑫[1] 谭春雷[1] 张学新[1] CHANG Liang;WANG Xin;TAN Chun-lei;ZHANG Xue-xin(Department of Neurosurgery,the Third Affiliated Hospital of Harbin Medical University,Harbin 150086,China)

机构地区：[1]哈尔滨医科大学附属第三医院神经外科,哈尔滨150081

出　　处：《临床误诊误治》2018年第6期91-95,共5页Clinical Misdiagnosis & Mistherapy

基　　金：黑龙江教育厅科学技术研究项目(12541467)

摘　　要：目的探讨miR-128对胶质母细胞瘤U87细胞侵袭性的影响及其作用机制。方法采用RTPCR法测定胶质母细胞瘤U87细胞和SHG-44细胞中miR-128的表达情况,应用Transwell侵袭和迁移实验检测U87细胞的侵袭性,采用荧光素酶报告基因分析法检测miR-128调节的靶基因,应用Western blot分析法检测MMP-7蛋白的表达情况,并与阴性对照组(NC组)进行比较。结果 miR-128在SHG-44细胞、U87细胞中的表达量分别为1.00±0.13、0.52±0.16,差异有统计学意义(P<0.05)。与NC组比较,肿瘤细胞高表达miR-128时两组穿膜细胞数分别为445±23、313±59,差异有统计学意义(P<0.05)。与NC组比较,肿瘤细胞过度表达miR-128时,MMP-7蛋白表达明显减少,差异有统计学意义(P<0.05)。结论 miR-128通过负性调控MMP-7蛋白的表达以抑制U87细胞的侵袭,提示miR-128可作为临床靶向治疗胶质母细胞瘤的关键。Objective To investigate effects of miR-128 on invasion of glioblastoma U87 cells and its molecular mechanism. Methods Reverse transcription-quantitative polymerase chain reaction（ RT-PCR） was used to measure the expression levels of miR-128 in glioblastoma U87 cells and SHG-44 cells. Migration and invasion of U87 cells were analyzed by transwell migration and invasion assays. Luciferase reporter assay was employed to detect cell target genes of miR-128,while western blot analysis was performed to verify the expression of MMP-7. Results The results showed that the expressions of miR-128 in SHG-44 cells and U87 cells were 1. 00 ± 0. 13 and 0. 52 ± 0. 16 respectively,and the difference was statistically significant（ P〈0. 05）. Compared with the NC group,the number of invasive cells was 445 ± 23 and 313 ± 59 respectively,and the difference was statistically significant（ P〈0. 05）.Compared with the NC group,when the tumor cells overexpressed miR-128,the expression of MMP-7 protein decreased significantly,revealing significant difference（ P〈0. 05）. Conclusion The miR-128 could inhibit tumor invasion and migration in U87 cells by negatively regulating MMP-7 protein and may be identified as a potential therapeutic target of glioblastoma.

关 键 词：胶质母细胞瘤 微RNAS 侵袭 肿瘤 

分 类 号：R730.264[医药卫生—肿瘤]