基于目标诱导银沉积的可视化检测方法的构建及其应用  

Construction and Application of Target-induced Silver Deposition Based Colorimetric Assay

作　　者：字琴江 王瑾 曹秋娥 周川华 ZI Qin-Jiang;WANG Jin;CAO Qiu-E;ZHOU Chuan-Hua(School of Chemical Science and Technology,Key Laboratory of Medicinal Chemistry for Natural Resouree（Ministry of Education）,Yunnan University,Kunming 650091,China;Eryuan Instritute for Food and Drug Control,Duli 671299,China)

机构地区：[1]云南大学化学科学与工程学院自然资源药物化学教育部重点实验室,昆明650091 [2]洱源县食品药品检验检测院,大理671299

出　　处：《分析化学》2018年第8期1215-1221,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21505119;21465025);云南省自然科学基金项目(No.2015FD002)资助~~

摘　　要：制备稳定且易于修饰的纳米金是构建基于金纳米颗粒可视化分析方法的关键。本研究以谷胱甘肽(GSH)为配体,分别采用一步法和两步法制备了两种纳米金颗粒,并对这两种纳米金的粒径、胶体稳定性等性能进行考察,发现两步法合成的纳米金胶体稳定性更好。在此基础上,采用两步法合成的纳米金作为晶种和催化剂,建立了一种基于金属纳米颗粒的非聚集可视化检测抗坏血酸(AA)的方法。此方法结合了目标诱导银沉积反应和纳米银大的摩尔消光系数,利用AA在纳米金的存在下将Ag^+还原成银单质所导致的检测液颜色变化及纳米银在410 nm处的吸光度变化,对AA进行定性以及定量检测,检出限为0.75μmol/L(S/N=3)。此方法具有简单、快速、灵敏、所需仪器设备简单、特异性好、抗干扰能力强等优点,可用于复杂样品中AA的高灵敏检测,在生物检测方面具有良好的应用前景。Preparation of gold nanoparticles（ AuNPs） with good stability is the key to construct metal nanoparticles based colorimetric assay method. Two kinds of AuNPs with glutathione as ligand were prepared using the one-step method and two-step method respectively. The particle size and colloid stability of the asprepared Au NPs were compared. The two-step method prepared Au NPs was more stable. Then,a nonaggregation colorimetric strategy for detection of ascorbic acid（ AA） based on metal nanoparticles was constructed using the Au NPs prepared by two-step method as seeds and catalyst. This strategy combined the target-induced silver deposition reaction and the extraordinarily high molar extinction coefficient of silver nanoparticles,which exhibited high detection sensitivity and good specificity. The presence of AA could reduce Ag＋to Ag0 on the surface of Au NPs,and the resulting color change of the detection solution and the A410 was used for AA detection. The detection limit was as low as 0.75 μmol/L（ S/N = 3）. In brief,a simple,fast and sensitive colorimetric assay method for detection of AA with good specificity and strong anti-interference ability was proposed. This method was applied to AA detection in real complex samples,which showed great prospect in bioanalytical applications.

关 键 词：纳米金 抗坏血酸 目标诱导银沉积 可视化检测 

分 类 号：O657.3[理学—分析化学] TB383.1[理学—化学]