菝葜皂苷对胃癌BGC-823细胞Bcl-2、Bax凋亡蛋白表达影响的实验研究  

Study on the Effect of Sarsasapogenin to Apoptosis of Bax and Bcl-2 Protein Expression of Human Gastric Cancer Lines BGC-823

作　　者：廖子君[1] 郑琪[1] 赵征[1] 孙海凤 龙婧 陈勇 LIAO Zi-jun, ZHENG Qi, ZHAO Zheng, SUN Hai-feng, LONG Jing, CHEN Yong(Affiliated Tumor Ho6pital of Shannxi Provincial, Medical College of Xi＇an Jiaotong University, Xi ＇an 710061, Chin)

机构地区：[1]西安交通大学医学院附属陕西省肿瘤医院,陕西西安710061

出　　处：《中国医药指南》2018年第18期1-2,共2页Guide of China Medicine

基　　金：陕西省卫生厅科学研究基金(06D09)

摘　　要：目的观察不同浓度菝葜皂苷元对体外培养的胃癌BGC-823细胞凋亡相关蛋白Bax、Bcl-2表达的影响。方法采用免疫组化方法,检测BGC-823细胞经不同浓度(12.5、25、50μg/mL)的菝葜皂苷元处理后Bcl-2、Bax的表达情况。结果随着菝葜皂苷元浓度的增加,与对照组比较,BGC-823细胞Bax表达逐渐增加,不同浓度组之间差异有统计学差异(P<0.05),而Bcl-2表达逐渐减少(P<0.05)。结论菝葜皂苷元可诱导BGC-823细胞凋亡,上调Bax蛋白水平与下调Bcl-2蛋白水平。Objective To investigate the effects of different concentrations of sarsasapogenin on apoptosis of Bax and Bcl-2 apoptosis-related protein expression of human gastric cancer cell line BGC-823 cultured in vitro. Methods The BGC-823 cells were treated by sarsasapogenin in different concentrations, Flow cytometry was used to assay the effects of sarsasapogenin on apoptosis and immunocytochemical method was used to assay Bax, Bcl-2 protein expression. Results The apoptosis rate of BGC-823 cell were significantly augmented and bax expressions increased and Bcl-2 expressions decreased while the concentration and duration of sarsasapogenin increased（P 0.05） respectively. Conclusion Sarsasapogenin can efficiently induce apoptosis of BGC-823 cell, up-regulate Bax and down-regulate Bcl-2 protein expression which are the possible mechanisms for its anti-cancer effect.

关 键 词：菝葜皂苷元 细胞株BGC-823 凋亡相关蛋白 

分 类 号：R735.2[医药卫生—肿瘤]