超临界流体色谱与气相色谱-质谱联用测定卷烟主流烟气中醛酮类化合物的研究  

Determination of Aldehydes and Ketones Compounds in Mainstream Cigarette Smoke by Supercritical Fluid Chromatography and Gas Chromatography-Mass Spectrometry

作　　者：崔婷惠 李恒 唐石云[1] 刘春波 高晓华 李振杰 张凤梅 杨光宇 蒋薇 刘志华 缪明明 何沛 申钦鹏 CUI Ting-hui;LI Heng;TANG Shi-yun;LIU Chun-bo;GAO Xiao-hua;LI Zhen-jie;ZHANG Feng-mei;YANG Guang-yu;JIANG Wei;LIU Zhi-hua;MIAO Ming-ming;HE Pei;SHEN Qin-peng(Key Laboratory of Tobacco Chemistry of Yunnan Province,Research and Development of Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,China;Academy of Chemical Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,云南省烟草化学重点实验室,云南昆明650231 [2]昆明理工大学化学工程学院,云南昆明650500

出　　处：《分析测试学报》2018年第7期766-771,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21365026); 云南省科学技术厅科技项目(2014FD078,2016CP021); 云南中烟工业有限责任公司项目(2018JC04,2017CP04)

摘　　要：建立了一种卷烟主流烟气中醛酮类香气化合物的超临界流体色谱/气相色谱-质谱测定方法。将捕集卷烟主流烟气粒相物的剑桥滤片用超临界流体萃取醛酮类香气成分后,通过配备高效分离柱的超临界流体色谱(SFC)先对香气成分进行划段,分离后的各段馏分再用GC-MS分析。结果表明:经SFC分离后的各段化合物之间基本不重叠,解决了GC-MS峰容量有限的问题,且分离出的醛酮类香气成分数量远多于直接进样分析法;样品直接进样后经GC-MS分析仅鉴定出29种醛酮类化合物,其中醛类7种、酮类22种;而经SFC分段后再用GC-MS分析,可鉴定出57种醛酮类化合物,包括13种醛类和44种酮类。3种不同焦油量卷烟主流烟气中醛酮类化合物种类和含量存在较大差异,每种卷烟主流烟气中分别含有不同特有醛酮类化合物。该研究为卷烟主流烟气中醛酮类香气成分的测定提供了新方法。To resolve the chromatographic column capacity of gas chromatography-mass spectrometry（GC-MS）,a GC-MS method with supercritical fluid chromatography（SFC） was developed for the determination of carbonyl compounds in mainstream cigarette smoke. The total particulate matter in mainstream cigarette smoke was collected with a Cambridge filter pad,and the carbonyl compounds were extracted with a supercritical fluid extractor,then the extract was separated and divided into 3 fractions by SFC method,and each fraction was analyzed by GC-MS. The results showed that the overlapping chromatographic peaks were avoided,and the chromatographic column capacity of GC-MS was improved. Therefore,the kinds of aldehydes and ketones aromatic components in mainstream cigarette smoke determined by the developed method were far more than that without SFC separation.Only 29 kinds of aldehydes and ketones compounds,including 7 kinds of aldehydes and 22 kinds of ketones,were identified without SFC separation, while 57 kinds of aldehydes and ketones compounds,including 13 kinds of aldehydes and 44 kinds of ketones,were determined using the devel-oped method. There were great differences in the species and contents of aldehydes and ketones in mainstream smoke of three cigarettes. Each cigarette contained different specific aldehydes and ketones in mainstream cigarette smoke. This study provided a new method for the determination of aldehydes and ketones in cigarette mainstream smoke.

关 键 词：超临界流体色谱(SFC) 气相色谱-质谱法(GC-MS) 卷烟主流烟气 香气成分 醛酮类化合物 

分 类 号：O657.63[理学—分析化学] O622.4[理学—化学]