免标记自增强电化学发光免疫传感器超灵敏检测河豚毒素  

A Label-free Self-enhanced Electrochemiluminescence Immunosensor for Ultrasensitive Detection of Tetrodotoxin

作　　者：刘媛 王邃[1] LIU Yuan;WANGSui(Ningbo Municipal Key Laboratory and Cultivation Base of Novel Functional Materials and Their Preparations,College of Material Science and Chemical Engineering,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学材料科学与化学工程学院,宁波市新型功能材料及其制备科学国家重点实验室培育基地,浙江宁波315211

出　　处：《分析测试学报》2018年第6期676-681,共6页Journal of Instrumental Analysis

基　　金：浙江省自然科学基金公益技术项目(LGF18B070002); 宁波市自然科学基金(2017A610231); 宁波大学王宽诚幸福基金资助项目

摘　　要：以Nafion固定钌联吡啶Au纳米颗粒(Ru(bpy)2+3-Au NPs)于玻碳电极(GCE),借助Au—N共价键固定河豚毒素抗体(anti-TTX),通过抗原抗体的特异性结合构建了超灵敏检测河豚毒素(TTX)的免标记自增强电化学发光(ECL)免疫传感器。当目标物TTX被特异性捕获到修饰电极表面后,可以作为Ru(bpy)2+3的共反应物增强ECL信号。与共反应物存在于溶液中或标记于二抗上相比,其优点在于发光体和共反应物之间的电子转移距离更短,可有效改善电化学发光效率,降低实验成本。最佳条件下,TTX质量浓度在0.01~1 000μg·L-1范围内与ECL信号呈良好的线性关系,检出限(LOD)为0.01μg·L-1。此免标记自增强型ECL免疫传感器显示出优异的稳定性、重现性和灵敏度,对实际样品中TTX的回收率为98.0%~104.0%,表明其具有较好的实用价值。A label-free self-enhanced electrochemiluminescence（ ECL） immunosensor was fabricated for the ultrasensitive detection of tetrodotoxin（ TTX） based on immobilizing tris（ 2,2＇-bipyridyl）ruthenium（ Ⅱ）-Au nanoparticles（ Ru（bpy）_3^（2＋）-Au NPs） composites to glassy carbon electrode（ GCE） with Nafion. In this strategy,an anti-TTX was able to fix on the electrode surface via Au-N chemical bond for realizing the specific capture toward the target. Meanwhile,TTX could be used as the co-reactant of Ru（bpy）_3^（2＋） to promote the ECL intensity. The advantage of this approach is that the electron transfer distance between the emitter and the co-reactant is shorter,and the ECL efficiency is improved and the experimental cost is reduced compared with that in the presence of co-reactant in solution or labeling of second antibody. Under the optimum conditions,the change of ECL intensity demonstrated a linear relationship with TTX concentration in the range of 0. 01-1 000 μg·L-1,with a limit of detection（ LOD） down to 0. 01 μg·L-1. This label-free self-enhanced ECL immunosensor exhibited an excellent performance with good stability,reproducibility and sensitivity. The recoveries for TTX in real samples were in the range of 98. 0%-104. 0%,indicated it has good practical value.

关 键 词：电化学发光 免疫传感器 河豚毒素 Nafion-Ru(bpy)32+-AuNPs复合物 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]