呼出气VOCs在上呼吸道感染病患与健康人间的异同  

Differences in Exhaled VOCs from Patients with Upper Respiratory Tract Infection and Healthy Ones

作　　者：王珏 郑云昊 刘兆荣[1] 要茂盛[1] 李晓光[2] 庄嘉 彭望远 WANG Jue;ZHENG Yunhao;LIU Zhaorong;YAO Maosheng;LI Xiaoguang;ZHUANG Jia;PENG Wangyuan(College of Environmental Science and Engineering,Peking University,Beijing 100871;Department of Infectious Diseases,Peking University Third Hospital,Beijing 100191)

机构地区：[1]北京大学环境科学与工程学院,北京100871 [2]北京大学第三医院感染疾病科,北京100191

出　　处：《北京大学学报（自然科学版）》2018年第4期807-814,共8页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家重点基础研究发展计划(2016YFC0207102)资助

摘　　要：研究健康人与病患呼出气VOCs成分的异同,为上呼吸道感染分型的无创快速诊断提供信息。采集60例上呼吸道感染患者、30例健康人呼出气,利用GC-MS定量检测97种VOCs,分析健康人及病患呼出气中烷烃、烯烃、卤代烃、含氧烃、芳香烃等物质的分布特点,并利用ANOVA-PCA,分析室内空气以及健康人和病患呼出气中VOCs的异同。结果表明,异戊二烯浓度在病患、健康人呼出气及室内空气中均有显著差异(P<0.05),且呈现病人>健康人>室内空气的特点;正戊烷浓度在病患组显著高于健康人组(P<0.05);丙醛浓度在细菌性上感组显著高于健康组(P=0.019)。PCA分析结果显示,健康人与上呼吸道感染病患呼出气差异明显,不同类型病患呼出气VOCs没有明显差异。Analysis of exhaled VOCs from health and patients may help building association between VOCs and different diseases condition, which could offer a possibility of noninvasive monitoring of disease. VOCs were collected in 30 healthy subjects and 60 patients with upper respiratory tract infection. Analysis of 97 VOCs was performed by gas chromatography and mass spectrometry（GC-MS）. The concentration of alkanes, alkenes, halogenated hydrocarbon, oxygenated hydrocarbons and aromatic hydrocarbons in health and patients breath was analyzed. Analysis of variance（ANOVA）, principal component analysis（PCA） were used to analyze the VOC differences from patients and healthy ones. The results show that the concentration of isoprene differ among patients, healthy ones and indoor air（P〈0.05）. Patients show higher concentration of n-pentane compared to healthy ones（P〈0.05）. Patients with bacterial upper respiratory tract infection show higher concentration of propanal compared to healthy ones. The results of PCA show that there were significant VOC differences between patients with upper respiratory tract infection and healthy ones（P=0.019）, but no differences between bacterial and non-bacterial upper respiratory tract infection.

关 键 词：呼出气VOCs 上呼吸道感染 健康人 ANOVA-PCA 

分 类 号：X132[环境科学与工程—环境科学] R562[医药卫生—呼吸系统]