Hemin诱导K562细胞红系分化过程中BCR/ABL表达的变化  

Changes of BCR/ABL expression during erythroid differentiation of K562 induced by Hemin

作　　者：高锦程[1] 郭传娟 赵红丽[1] 洪珞珈[1] Gao Jincheng;Guo Chuanjuan;Zhao Hongli;Hong Luojia(Department of Hematology,the Fourth Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China;Shandong Province Yantai Nursing School,Shandong Yantai 264000,China)

机构地区：[1]哈尔滨医科大学附属第四医院血液科,黑龙江哈尔滨150001 [2]山东省烟台护士学校,山东烟台264000

出　　处：《现代肿瘤医学》2018年第15期2346-2349,共4页Journal of Modern Oncology

基　　金：黑龙江省教育厅科学技术研究面上项目(编号:12541299)

摘　　要：目的:观察氯化高铁血红素(Hemin)在诱导K562细胞红系分化过程中融合基因BCR/ABL表达的变化。方法:分别应用不同浓度的Hemin刺激K562细胞24 h后,光学显微镜下观察Hemin诱导前后细胞的形态变化,采用CCK8法检测0、10、20、30、40、50μmol/L的Hemin诱导24 h后细胞的增殖活性,并通过实时荧光定量PCR及蛋白质印迹分析分别从基因和蛋白水平检测各组BCR/ABL融合基因的表达。结果:经过不同浓度的Hemin诱导24 h后,细胞增殖活性降低,BCR/ABL融合基因的表达下调,且随着Hemin浓度的升高融合基因的表达量逐渐减低。结论:Hemin明显抑制BCR/ABL融合基因的表达,作用随着Hemin浓度的加大逐渐增强。Objective：To study the effects of Hemin on the expression of BCR/ABL in K562 cells．Methods：The K562 cells were stimulated by different concentrations of Hemin for 24 h，and the morphological changes of cells and cell proliferation were observed.And CCK8 method was used to detect the proliferation activity of K562 cells induced by 0，10，20，30，40 and 50 μmol/L for 24 h.The expression of BCR/ABL fusion gene in each group was detected by real-time fluorescence quantitative PCR and Western blotting respectively from the gene and protein levels．Results：After 24 h induction with different concentrations of Hemin，the cell proliferation activity was decreased，and the expression of BCR/ABL fusion gene was down-regulated，and the expression of fusion gene was gradually decreased with the increase of Hemin concentration.Conclusion：Hemin significantly inhibited the expression of BCR/ABL fusion gene，and its effect increased gradually with the increase of Hemin concentration.

关 键 词：氯化高铁血红素 K562细胞 BCR/ABL融合基因 

分 类 号：R733.72[医药卫生—肿瘤]