胰腺癌患者血循环miR-429的表达及其临床意义  

The expression of miR-429 in patients with pancreatic cancer and clinical significance

作　　者：刘棣[1] 宋玲琴[1] 张寅斌[1] 许朋 盛倩文 管海涛[1] 代志军[1] 张淑群[1] Liu Di;Song Lingqin;Zhang Yinbin;Xu Peng;Sheng Qianwen;Guan Haitao;Dai Zhijun;Zhang Shuqun(Department of Oncology,the Second Affiliated Hospital of Xi＇an Jiaotong University,Shaanxi Xi＇an 710004,China)

机构地区：[1]西安交通大学第二附属医院肿瘤科,陕西西安710004

出　　处：《现代肿瘤医学》2018年第15期2389-2392,共4页Journal of Modern Oncology

基　　金：中央高校基本科研业务费专项资金资助(编号:2014gjhz11;xjj2018131);陕西省科技厅重点研发计划一般项目资助(编号:2017SF-172);必康基金(编号:2017BIKANGJIJIN-020);西安交通大学第二附属医院院青年基金[编号:YJ(QN)201305]

摘　　要：目的:检测胰腺癌患者血浆miR-429表达水平,分析其表达与胰腺癌临床病理学特征的关系。方法:定量PCR检测BXPC-3、PANC-1细胞株以及3例胰腺癌组织中miR-429的表达水平。收集未经治疗的37例胰腺癌患者的血浆标本,采用实时定量PCR法检测miR-429表达水平,收集胰腺癌患者的临床病理学参数(年龄、临床分期、分化程度、组织类型及淋巴结转移情况),比较不同miR-429水平的临床病理参数分布差异。结果:相较于正常胰腺细胞、胰腺组织,miR-429在胰腺癌细胞株和癌组织中均处于低表达水平(P<0.05)。miR-429表达与肿瘤大小、TNM临床分期、神经浸润以及生存期密切相关,均有统计学差异(P<0.05)。结论:循环血miR-429的表达可能成为胰腺癌早期诊断、预后判断的指标。Objective：To investigate the expression of miR-429 in pancreatic cancer and explore the relationship between the expression of miR-429 and its clinical pathology features.Methods：The expression of miR-429 in BXPC-3,PANC-1 cells and pancreatic cancer tissues were inspected by Real-time PCR.Collect serum from 37 patients with pancreatic cancer.Real-time PCR was conducted to measure miR-429 expression in the serum of pancreatic cancer patients.The relationship between miR-429 expression and pathological features were analyzed.Results：miR-429 was observed down-regulated in pancreatic cancer cell lines,tissue and in patient＇s serum（P〈0.05）.The statistical analysis suggested that miR-429 was associated with tumor size,TNM stage,nerves infiltration and survival（P〈0.05）.Conclusion：The expression of miR-429 in serum could be a new biomarker for pancreatic cancer early diagnosis and predictor of prognosis.

关 键 词：miR-429 胰腺癌 早期诊断 

分 类 号：R735.9[医药卫生—肿瘤]