参附注射液对大鼠短暂性局灶性脑缺血损伤的治疗时间窗  

Study of therapeutic time window for Shenfu solution in transient focal cerebral ischemic injury in rats

作　　者：郑玉[1] 熊利泽[1] 朱正华[1] 王强[1] 陈绍洋[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2002年第15期1353-1356,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (3 0 170 90 7)

摘　　要：目的　探讨参附注射液对局灶性脑缺血性损伤的治疗时间窗 .方法　 80只雄性 SD大鼠 ,随机分为 8组 (每组 n=10 ) :对照组 ,即单纯缺血再灌注组 ;参附注射液治疗 A～ G组 ,分别于缺血再灌注后 0 ,0 .5 ,1.0 ,1.5 ,2 .0 ,4 .0和 6 .0 h经腹腔注射参附注射液 ,剂量为 10 m L·kg- 1 .用右侧颈内动脉线栓法致大脑中动脉阻闭 12 0 min,拔出尼龙线即刻为再灌注时间 .观察再灌注后 2 4 h时神经功能损害改变并评分 ,2 4 h时处死动物 ,取大脑行 TTC染色以测量脑梗死容积 .结果　 2 4 h时神经功能损害评分 ,A～ F组分别为 :0 .5± 0 .3,0 .5± 0 .4 ,0 .6± 0 .5 ,0 .8± 0 .4 ,0 .8± 0 .3和 0 .9± 0 .3,明显低于对照组 (2 .1± 0 .8)及 G组 (1.5± 0 .7) (P<0 .0 5 ) .脑梗死容积 A～ F组分别为(46 .4± 2 6 .1) ,(6 0 .2± 2 5 .8) ,(6 7.2± 36 .7) ,(76 .0± 39.7) ,(94 .1± 34.9)和 (98.4± 35 .3) mm3;均明显小于对照组 (182 .6± 39.6 ) mm3及 G组 (139.1± 4 2 .8) mm3(P<0 .0 5 ) ,A～ F组间 2 4 h时神经功能损害评分和梗死容积无差别 (P>0 .0 5 ) .结论　参附注射液对局灶性脑缺血再灌注损伤的最佳治疗时机在缺血后 4AIM To investigate the therapeutic time window for Shenfu solution in transient focal cerebral ischemic injury in rats. METHODS 80 male SD rats weighing 280~350 g were randomized into 8 groups ( n =10 in each group): Control group, receiving no pharmacological intervention, Groups A^G, receiving intraperitoneal injection of Shenfu solution (10 mL·kg -1 ) at 0, 0.5, 1.0, 1.5, 2.0, 4.0 and 6.0 h respectively after 120 min middle cerebral artery occlusion (MCAO) with 3 0 nylon monofilament via the right internal carotid artery. The neurological outcomes were evaluated 24 h after the reperfusion. The brain infarct volumes were then assessed by TTC staining. RESULTS The neurological deficit scores (NDS) for Groups A^F 24 h after the reperfusion were 0.5±0.3, 0.5±0.4, 0.6±0.5, 0.8±0.4, 0.8 ±0.3 and 0.9±0.3, respectively, which were obviously lower than those of the control group (2.1±0.8) and Group G ( 1.5 ±0.7) ( P <0.05). The infarct volumes of the control group (182.6±39.6) mm 3 and Group G (139.1±42.8) mm 3 were significantly larger than those of Groups A^F ( 46.4 ±26.1), (60.2±25.8), (67.2±36.7), (76.0±39.7), (94.1±34.9) and (98.4±35.3) mm 3, respectively ( P <0.05) 24 h after the reperfusion. There were no differences between Groups A^F in both NDS and infarct volume ( P >0.05). CONCLUSION Therapeutic time window for Shenfu solution in transient focal cerebral ischemic injury lasts for at least 4 h.

关 键 词：参附注射液 大鼠 短暂性局灶性脑缺血损伤 治疗时间窗 

分 类 号：R743.31[医药卫生—神经病学与精神病学]