钠离子通道在异氟醚预处理诱导的脑保护中的作用  

Role of sodium channel in the neuroprotective effect induced by preconditioning with isoflurane in rats

作　　者：刘艳红[1] 熊利泽[1] 陈绍洋[1] 郑玉[1] 路志宏[1] 胡胜[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2002年第15期1366-1368,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (3 0 170 90 7)

摘　　要：目的　探讨钠离子通道阻断剂利多卡因在异氟醚预处理所诱导的脑保护中的作用 .方法　 4 0只雄性 SD大鼠 ,随机分为 4组 (每组 n=10 ) ,即空白对照组 ;利多卡因对照组 ,每日给予利多卡因 (5 mg· kg- 1 ,ip)连续 5 d;异氟醚处理组 ,每日给予 15 m L· L- 1异氟醚吸入 1h,连续 5 d;利多卡因加异氟醚处理组 ,在每次异氟醚处理之前给予利多卡因 (5 mg·kg- 1 ,ip) ,余处理同异氟醚处理组 .最后一次处理 2 4 h后采用颈内动脉尼龙线线栓法致右大脑中动脉栓塞 (12 0 min)模型 ,观察再灌注后 2 4 h时神经功能损害并取大脑行 TTC染色以测量脑梗死容积 .结果　再灌注 2 4 h时神经功能损害评分及脑梗死容积 ,异氟醚处理组均明显小于其余 3组 (P<0 .0 5 ) ,而利多卡因加异氟醚处理组及利多卡因处理组的神经功能学评分及脑梗死容积与空白对照组均无明显差异 (P>0 .0 5 ) .结论　本实验进一步证明了多次吸入异氟醚可诱导产生脑保护作用 .此作用可被利多卡因阻断 。AIM To investigate the effects of lidocaine, a sodium channel blocker, on the ischemic tolerance induced byrepeated brief isoflurane anesthesia in the focal cerebral ischemia in rats. METHODS Forty male Sprague Dawley rats were randomly assigned to four groups ( n =10 each): Control Group, animals without treatment; Lidocaine Group, animals intraperitoneally injected with lidocaine (5 mg·kg -1 , ip.) once a day for 5 days and Isoflurane Group and Isoflurane+Lidocaine Group, animals given 15 mL·L -1 isoflurane 1 h per day for 5 days, while those in the Isoflurane+Lidocaine Group received lidocaine (5 mg·kg -1 ip.) just before each isoflurane pretreatment. 24 h after the last pretreatment, transient MCAO (120 min) was induced. The neurological outcome was evaluated 24 h after the reperfusion. The infarct volume was then assessed with TTC staining after the neurological outcome evaluation. RESULTS The neurologic deficit scores in the Isoflurane Group were lower than those of the other three groups ( P <0.05). There was no statistical difference between the Control, Isoflurane+Lidocaine and Lidocaine Groups. The infarct volume in the Isoflurane Group was less than those of the other three groups ( P < 0.05 ). CONCLUSION The present study was demonstrated that repeated isoflurane anesthesia is able to induce ischemic tolerance in transient MCAO rats. This effect can be blocked by lidocaine, which suggests the sodium channel is involved in the formation of ischemic tolerance induced by isoflurane preconditioning.

关 键 词：钠离子通道 异氟醚 预处理 脑保护 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R614[医药卫生—临床医学]