TEPN对2型糖尿病大鼠的降血糖作用及其机制  

Hypoglycemic effect and mechanism of TEPN on type 2 diabetes in rats

作　　者：韩金川 任文彦 王红英[2] HAN Jin-chuan;REN Wen-yan;WANG Hong-ying(Cardiology Department,the Hospital of Qishan County,Baoji 722400;Pharmacology Department,Basic Medical College ofXi＇an Jiaotong University,Xi＇an 710061,China)

机构地区：[1]陕西省岐山县医院心内科,陕西宝鸡722400 [2]西安交通大学基础医学院药理学系,陕西西安710061

出　　处：《临床医学研究与实践》2018年第22期7-8,13,共3页Clinical Research and Practice

摘　　要：目的研究替普瑞酮(TEPN)对2型糖尿病的降血糖作用及其机制。方法给予2型糖尿病模型大鼠口服TEPN(75、150 mg/kg)和二甲双胍(45 mg/kg)4周,观察动物空腹血糖、胰岛素、胆固醇、甘油三酯、游离脂肪酸、热休克蛋白(HSP)70以及肝脏、胰腺、脂肪和肌肉组织HSP70与肝脏JNK、p-JNK的表达水平。结果与模型对照组比较,TEPN给药组和二甲双胍组动物的空腹血糖、胰岛素、胆固醇、甘油三酯、游离脂肪酸明显更低,血清HSP70水平明显更高(P<0.05)。与模型对照组比较,TEPN给药组动物的肝脏、胰腺和肌肉组织中HSP70的表达水平明显更高,肝脏组织中JNK和p-JNK的表达水平明显更低(P<0.05)。结论 TEPN对2型糖尿病大鼠模型有降血糖作用,其机制可能与提高血清和组织HSP70水平、抑制JNK信号转导通路有关。Objective To study the hypoglycemic effect and mechanism of teprenone （TEPN） on type 2 diabetes mellitusin rat. Methods The type 2 diabetes mellitus model rats were given TEPN （75, 150 mg/kg） and metformin （45 mg/kg）orally for 4 weeks. The levels of fasting glucose, insulin cholesterol, triglycerides, free fatty acid, heat shock protein （HSP）70 as well as the expression levels of HSP70 in liver, pancreas, fat and muscle and the expression levels of JNK and p-JNK in liver were observed. Results Compared with the model control group, the levels of fasting glucose, insulin,cholesterol, triglycerides, free fatty acid significantly reduced and serum HSP70 increased in the TEPN group andmetformin group （P〈0.05）. Compared with the model control group, the expression levels of HSP70 in the liver, pancreasand muscle significantly improved, and the expression levels of JNK and p-JNK in the liver significantly reduced in theTEPN group（P〈0.05）. Conclusion TEPN have hypoglycemic effect on type 2 diabetic mellitus rats, its mechanism may berelated to the increase of serum and tissue HSP70 levels and inhibition of JNK signaling pathway.

关 键 词：TEPN 2型糖尿病 降血糖作用 HSP70 大鼠 

分 类 号：R285[医药卫生—中药学]