改良的QuEChERS结合气相色谱-质谱法测定新鲜水果和肉中肉桂醛残留  

Determination of cinnamaldehyde residues in fresh fruits and meats by modified QuEChERS combined with gas chromatography-tandem mass spectrometry

作　　者：刘萤[1] 张蓉[1] 崔凤云[1] 韩深[1] LIU Ying, ZHANG Rong, CUI Fengyun, HAN Shen(Mspection and Quarantine Technical Center of Beijing Entry-Exit htspection and Quarantine Bureau, Beijing 100025, Chin)

机构地区：[1]北京出入境检验检疫局检验检疫技术中心,北京100026

出　　处：《色谱》2018年第6期541-546,共6页Chinese Journal of Chromatography

基　　金：食品安全国家标准制定项目(spaq-2016-179)~~

摘　　要：建立了改良的QuEChERS技术结合气相色谱-质谱检测水果和肉中肉桂醛残留量的定性定量分析方法。试样用乙酸乙酯溶液均质提取,经N-丙基乙二胺(PSA)、石墨化炭黑(GCB)、C18固相吸附剂净化,在电子轰击电离(EI)源、选择离子监测(SIM)模式下检测,外标法定量。肉桂醛在0.01～0.5 mg/L范围内线性关系良好,相关系数大于0.999。水果和鲜肉中肉桂醛的定量限分别为0.5 mg/kg和1.0 mg/kg。在3个添加水平下,肉桂醛的加标回收率为81.9%～104.5%,相对标准偏差(RSD,n=6)为1.1%～8.6%。该方法操作简单,净化效果好。A method for the determination of cinnamaldehyde residues in fresh fruits and meats by gas chromatography-tandem mass spectrometry（ GC-MS） coupled with modified QuEChERS was developed. The samples were extracted with ethyl acetate. Solid phase extraction agents such as graphitized carbon black（ GCB）,primary-secondary amine（ PSA） and octadecyl bonded silica（ C18） were used for adsorption and purification. The extracts were detected by electron impact ionization in selected ion monitoring（ SIM） mode and quantified using external standard method. The results revealed correlation coefficients（ R2） greater than 0. 999 in the range of0. 01-0. 5 mg/L. The limits of quantification（ LOQs） were 0. 05 to 0. 1 mg/kg. The recoveries at the three spiked levels ranged from 81. 9% to 104. 5% with the relative standard deviations（ RSDs） ranging from 1. 1% to 8. 6%. The proposed method is efficient,sensitive,reliable,and is suitable for the trace determination of the cinnamaldehyde residues in fresh fruits and meats.

关 键 词：气相色谱-质谱 QUECHERS 肉桂醛 水果 肉 

分 类 号：O658[理学—分析化学]