miR-23a和miR-125b在进展期结直肠癌中表达水平的研究  

The levels of miR-23a and miR-125b in the expression of advanced stages of colorectal cancer: an observational study

作　　者：李志发[1] 陈戎[1] 吴小兵[1] 黎淑玲[1] LI Zhi-fa;CHEN Rong;WU Xiao-bing;LI Shu-ling(Department of Gastrointestinal Surgery,The Third Affiliated Hospital of Guangzhou Medical University,Guangdong Province,Guangzhou 510150,P.R.China)

机构地区：[1]广东省广州市广州医科大学附属第三医院胃肠外科,广东广州510150

出　　处：《消化肿瘤杂志（电子版）》2018年第1期25-28,共4页Journal of Digestive Oncology(Electronic Version)

基　　金：MicroRNA-490-3p靶向TGFβR1对结直肠癌侵袭转移的分子机制研究(2016114104437517)

摘　　要：目的分析miR-23a和miR-125b作为结直肠癌(Colorectal Cancer,CRC)诊断标记的潜能,并分析肿瘤分期对miR-23a和miR-125b表达水平的影响。方法采用实时定量PCR(RT-q PCR)分析miR-23a在CRC患者肿瘤和瘤旁组织之间的表达谱,并分别分析miR-23a和miR-125b在Ⅱ、Ⅲ期CRC中的表达水平。结果 miR-23a和miR-125b在Ⅱ、Ⅲ期CRC患者及混合样本中的均为下调表达,且miR-125b在三组结直肠中下调表达均具有统计学意义(P<0.05)。结论 miR-23a和miR-125b具有作为结直肠癌诊断标记的潜能,但肿瘤分期对miR-23a和miR-125b的表达水平不一定具有明显影响。Objective Aim to confirm miR-23 a and miR-125 b as potential diagnostic markers of colorectal cancer（CRC）, and evaluate the effect of tumor staging using the miR-23 a and miR-125 b expression level. Method Real-time quantitative PCR（RT-PCR） analysis of the expression profile of miR-23 a in the CRC tumors and adjacent tumor organizations, and were analyzed miR-23 a and miR-125 b expression levels at stage Ⅱ, Ⅲ CRC. Result Both miR-23 a and miR-125 b were down-regulated in stage Ⅱ, Ⅲ CRC tumors and mixed samples, and miR-125 b was down-regulated in all three groups（P〈0.05）. Conclusions Mi R-23 a and miR-125 b are potentially diagnostic markers for CRC, but the tumor staging does not necessarily have a significant effect on the expression levels of miR-23 a and miR-125 b.

关 键 词：结直肠肿瘤 miR-23a miR-125b 生物标记 诊断标记 

分 类 号：R735.34[医药卫生—肿瘤]