Wnt/β-catenin信号通路对套细胞淋巴瘤中细胞增殖、侵袭性生长的影响  

Effects of Wnt/β-catenin signaling pathway on the proliferation and invasive growth of cells in mantle cell lymphoma

作　　者：张文迪 余肖 蔡在胜 ZHANG Wen-di;YU Xiao;CAI Zai-sheng(Pediatrics Department,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《海南医学院学报》2018年第10期1045-1048,共4页Journal of Hainan Medical University

基　　金：国家重点基础研究发展项目(973计划)(2005CB5225007);卫生部临床学科重点项目2010-2012;"十一五"国家科技支撑计划(2006BAI05A07)~~

摘　　要：目的:研究Wnt/β-catenin信号通路对套细胞淋巴瘤中细胞增殖、侵袭性生长的影响。方法:选择2015年3月~2017年5月期间华中科技大学同济医学院附属同济医院经淋巴结活检诊断为套细胞淋巴瘤的患者作为研究的淋巴瘤组,同期经淋巴结活检诊断为反应性增生的患者作为对照组。采用酶联免疫吸附试剂盒测定淋巴结组织中Wnt/β-catenin信号通路分子的蛋白表达量,采用荧光定量PCR试剂盒测定增殖基因、侵袭基因的mRNA表达量。结果:淋巴瘤组患者淋巴结组织中β-catenin、pGSK-3β、Tcf/Lef的蛋白含量以及C-myc、CyclinD1、Est-1、MMP9、MMP26的mRNA表达量显著高于对照组,DKK1、WIF-1的蛋白含量以及Bax、Apaf1、Caspase-3、TNFAIP3、TIMP2、TIMP4的mRNA表达量显著低于对照组。淋巴瘤组织中C-myc、CyclinD1、Est-1、MMP9、MMP26的mRNA表达量与β-catenin、p-GSK-3β、Tcf/Lef的蛋白含量呈正相关,与DKK1、WIF-1的蛋白含量呈负相关;Bax、Apaf1、Caspase-3、TNFAIP3、TIMP2、TIMP4的mRNA表达量与β-catenin、p-GSK-3β、Tcf/Lef的蛋白含量呈负相关,与DKK1、WIF-1的蛋白含量呈正相关。结论:Wnt/β-catenin信号通路的激活能够促进套细胞淋巴瘤中细胞的增殖及侵袭性生长。Objective： To study the effects of Wnt/β-catenin signaling pathway on the proliferation and invasive growth of cells in mantle cell lymphoma. Methods： Patients who were diagnosed with mantle cell lymphoma by lymph node biopsy in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology between March 2015 and May 2017 were selected as lymphoma group of the research, and patients who were diagnosed with reactive hyperplasia by lymph node biopsy during the same period were selected as the control group. The protein expression of Wnt/β-catenin signaling pathway molecules in lymph node tissues were determined by enzyme-linked immunosorbent assay kit, and the mRNA expression of proliferation genes and invasion genes were determined by fluorescence quantitative PCR kit. Results： β-catenin, p-GSK-3β and Tcf/Lef protein levels as well as C-myc, CyclinD1, Est-1, MMP9 and MMP26 mRNA expression in lymph node tissues of lymphoma group were significantly higher than those of control group whereas DKK1 and WIF-1 protein levels as well as Bax, Apaf1, Caspase-3, TNFAIP3, TIMP2 and TIMP4 mRNA expression were significantly lower than those of control group （ P 〈0.05）. C-myc, CyclinD1, Est-1, MMP9 and MMP26 mRNA expression in lymphoma tissues were positively correlated with β-catenin, p-GSK-3β and Tcf/Lef protein levels, and negatively correlated with DKK1 and WIF-1 protein levels; Bax, Apaf1, Caspase-3, TNFAIP3, TIMP2 and TIMP4 mRNA expression were negatively correlated with β-catenin, p-GSK-3β and Tcf/Lef protein levels, and positively correlated with DKK1 and WIF-1 protein levels. Conclusion： The activation of Wnt/β-catenin signaling pathway can promote the proliferation and invasive growth of cells in mantle cell lymphoma.

关 键 词：套细胞淋巴瘤 WNT/Β-CATENIN信号通路 增殖 侵袭 

分 类 号：R733[医药卫生—肿瘤]