血清p-ANCA与sRAGE在溃疡性结肠炎中的表达及联合检测的诊断意义  

Expression of Serum p-ANCA and sRAGE in Ulcerative Colitis and Diagnostic Significance of Combined Test

作　　者：刘增福[1] 李庆芝[1] 葛红梅[2] 付景[1] 吴卫东[1] LIU Zeng-Ju;LI Qing-zhi;GE Hong-rnei;FU Jing;WU Wei-dong(Digestive System Department,Heze Municipal Hospital,Heze,Shandong Province,274031 China;Department of Neu rosurgery,Heze Municipal Hospital,Heze,Shandong Province,274031 China)

机构地区：[1]山东省菏泽市立医院消化内科,山东菏泽274031 [2]山东省菏泽市立医院神经外科,山东菏泽274031

出　　处：《中外医疗》2018年第17期11-13,25,共4页China & Foreign Medical Treatment

摘　　要：目的检测溃疡性结肠炎患者血清核周型抗中性粒细胞抗体(p-ANCA)、可溶性晚期糖基化终末产物受体(s RAGE)的表达情况,探讨p-ANCA和s RAGE作为溃汤性结肠炎患者辅助诊断指标的价值。方法方便收集该院2014年5月—2015年5月间就诊的68例溃疡性结肠炎患者(溃疡性结肠炎组)、42例非溃疡性结肠炎引起腹泻的患者(疾病对照组)、30名健康体检人群(对照组);用间接免疫荧光法(IIF)检测各组患者血清p-ANCA的表达,采用酶联免疫吸附试验(ELISA)法检测各组患者血清中s RAGE表达。结果溃疡性结肠炎组、疾病对照组和对照组的p-ANCA的阳性率分别为64.7%(44/68),7.1%(3/42)和0.0%(0/30),各组之间差异有统计学意义(χ2=17.52、21.71,P<0.01);溃疡性结肠炎组患者的s RAGE阳性率为51.5%(35/68)高于肠道疾病对照组16.7%(7/42)和对照组13.3%(4/30)(χ2=4.71、4.03,P<0.05);且p-ANCA与s RAGE表达呈正相关(r=0.784,P<0.01;r=0.822,P<0.01);两者联合检测,溃疡性结肠炎组的阳性率为64.8%。结论 p-ANCA和s RAGE可作为溃疡性结肠炎诊断时的辅助指标,具有一定的诊断意义,联合检测p-ANCA和s RAGE可以提高诊断的阳性率。Objective To test the expressions of p-ANCA and s RAGE of patients with ulcerative colitis and study the value of p-ANCA and s RAGE as the adjunctive diagnostic indexes. Methods 68 cases of patients with ulcerative colitis diagnosed in our hospital from Mary 2014 to Mary 2015（ulcerative colitis group）, 42 cases of patients with diarrhea due to nonulcerative colitis（disease control group） and 30 cases of healthy physical examination group（control group） were convenient collected, and the expressions of serum p-ANCA in each group was tested by IIF, and the serum s RAGE expression in each group was tested by the ELISA. Results The p-ANCA positive rates in the ulcerative colitis group, disease control group and control group were respectively 64.7%（44/68）, 7.1%（3/42）, 0.0%（0/30）, and the difference was statistically significant（χ2=17.52,21.71,P〈0.01）, and the s RAGE positive rate of patients in the ulcerative colitis group was higher than that in the disease control group and in the control group [51.5%（35/68） vs 16.7%（7/42）, 13.3%（4/30）]（χ2=4.71, 4.03,P〈0.05）, and the p-ANCA and s RAGE expression had a positive correlation（r=0.784, P〈0.01; r=0.822, P〈0.01）, and the positive rate of ulcerative colitis group was 64.8%. Conclusion The p-ANCA and s RAGE can be used as the adjunctive indexes in diagnosis of ulcerative colitis, with a certain diagnostic significance, and the combined test of p-ANCA and s RAGE can improve the diagnosis positive rate.

关 键 词：溃疡性结肠炎 P-ANCA s RAGE IIF ELISA 

分 类 号：R574[医药卫生—消化系统]