Yes相关蛋白与结肠癌病理因素的相关性  

Correlation of Yes-associated protein and the pathological factors of colon cancer

作　　者：高振文 杨淑萍 谢雪雪 宋伟[1] GAO Zhenwen;YANG Shuping;XIE Xuexue;SONG Wei(Department of Minimally Invasive Tumor Therapies,Shandong Provincial Hospital Affiliated to Shandong University,Jinan 250021,Shandong,China)

机构地区：[1]山东大学附属省立医院肿瘤微创综合治疗科,山东济南250021

出　　处：《山东大学学报（医学版）》2018年第7期57-64,共8页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81602332);山东省科技发展计划(2007GG30002024)

摘　　要：目的探讨新型转录共激活因子Yes相关蛋白(YAP)的表达与结肠癌临床病理因素及肿瘤转移的相关性。方法选取104例组织蜡块,其中80例为结肠癌组织,24例为结肠癌转移灶组织。应用免疫组织化学法检测结肠癌原发灶及其多器官转移灶中YAP及N-钙黏蛋白的表达,应用qRT-PCR和Western blotting检测YAP、N-钙黏蛋白及E-钙黏蛋白在6种结肠癌细胞中的表达。结果 N-钙黏蛋白在结肠癌原发灶组织中的阳性表达率高于结肠癌转移灶组织(P<0.05),而YAP阳性表达率的差异无统计学意义(P>0.05)。YAP与N-钙黏蛋白的共同表达仅在结肠癌原发灶中呈正相关(r=0.5003,P<0.001),在结肠癌转移灶中两者的表达没有相关性(r=0.0325,P>0.05)。YAP及N-钙黏蛋白的阳性表达与肿瘤浸润深度及远处转移有关(P<0.05),而与肿瘤发生部位、大体形态、组织学类型、分化程度、肿瘤大小、淋巴结转移及脉管内癌栓无关(P>0.05)。在SW480、SW620、SW1116、DLD-1、HCT116和HT29这6种细胞中,YAP表达量的差异无统计学意义(P>0.05),E-钙黏蛋白在HT29和SW1116中的表达显著高于其他细胞,N-钙黏蛋白在HCT116和SW620中表达最高。结论 YAP蛋白能够通过调控上皮-间质转化过程,促进结肠癌的发展及转移。Objective To investigate the expression of Yes-associated protein（ YAP）,a new transcriptional coactivator,and its correlation with the clincopathological factors and metastases of colon cancer. Methods A total of 104 paraffin embedded tissues were selected,including 80 colon cancer tissues and 24 metastatic tissues. The protein expressions of YAP and N-cadherin in the tissues were detected with immunohistochemical method. The expressions of YAP,E-cadherin and N-cadherin in six colon cancer cell lines were determined with qRT-PCR and Western blotting. Results There was no significant difference of YAP positive expression between the primary and metastatic colon cancer（ P〉0.05）,while the positive expression of N-cadherin was significantly higher in primary colon cancer than in the metastatic cancer（ P〈0.05）. There was positive correlation between YAP and N-cadherin in primary colon cancer（ r = 0.5003,P〈0.001）,but not in the metastatic cancer（ r = 0.0325,P〉0.05）. The positive expressions of YAP and N-cadherin were correlated with serosal invasion and distant metastases（ P〈 0. 05）,not with anatomical and histological type,differentiation,tumor size,lymph node metastases,microvascular invasion,left-sided and right-sided colon cancer（ P〉0.05）. In the six colorectal cancer cell lines（ SW480,SW620,SW1116,DLD-1,HT29 and HCT116）,there were no differences in the mRNA and protein expressions of YAP（ P〉0.05）. E-cadherin was highly expressed in HT29 andSW1116,and N-cadherin was highly expressed in HCT116 and SW620. Conclusion YAP can promote the development and metastasis of colon cancer by regulating the epithelial-mesenchymal transition.

关 键 词：Yes相关蛋白 结肠癌 上皮-间质转化 N-钙黏蛋白 原发灶 转移灶 

分 类 号：R735.3[医药卫生—肿瘤]