DKK3在胃癌组织中的表达及其与预后的关系  

The expression and prognostic significance of DKK3 in gastric cancer

作　　者：宋国栋[1] 王力[1] 沈洪[1] 李盟[1] 翟儒钧[1] 牛远杰[1] Song Guodong;Wang Li;Shen Hong;Li Meng;Zhai Rujun;Niu Yuanjie(Department of Gastrointestinal Surgery,the Second Hospital of Tianjin Medical University,Tianjin 300211,China)

机构地区：[1]天津医科大学第二医院胃肠外科,300211

出　　处：《中华普通外科杂志》2018年第7期585-588,共4页Chinese Journal of General Surgery

基　　金：天津市卫生和计划生育委员会科技基金资助项目（2015KZ096）

摘　　要：目的探讨胃癌组织中DKK3蛋白和mRNA的表达水平及其与患者预后的关系。方法应用免疫组织化学方法检测158例胃癌和癌旁组织中DKK3蛋白的表达水平，用免疫印迹和实时定量PCR检测76例胃癌和癌旁组织中DKK3蛋白和mRNA的表达水平。结果158例胃癌组织中DKK3阳性表达率低于癌旁组织（40．5％比55．1％,X^2=41．442，P=0．000）。76例胃癌组织中DKK3蛋白和mRNA的表达水平均低于癌旁组织（1．096±1．110比3．476±1．119,t=3．332．P=0．017；1．136±1．013比3．314±1．105，t=2．673，P=0．022）。DKK3的表达水平与胃癌的肿瘤浸润深度（P=0．006）、TNM分期（P=0．005）及淋巴结转移（P=0．009）密切相关，与胃癌患者的总生存率呈正相关（47．4％比28．0％，P=0．011），并且是其独立预后因素（P=0．016）。结论DKK3在胃癌组织中表达水平降低，并与胃癌患者的预后密切相关。Objective To study the expression of DKK3 protein and mRNA levels in gastric cancer and its correlation with the clinical prognosis of gastric cancer. Methods The expression of DKK3 protein in 158 gastric cancer specimens and paired adjacent non-cancerous tissues were detected by immunohistochemistry. The relationship between the protein expression and clinicopathological features was analyzed. Immunoblotting and real time quantitative PCR were used to examine the expression of DKK3 protein and mRNA levels in 76 paired fresh gastric cancer and adjacent non-cancerous tissues. Results The expression of DKK3 in gastric cancer tissues was lower than that in adjacent non-cancerous tissues （40. 5% vs. 55.1% ,X^2 = 41. 442, P = 0. 000）. DKK3 protein and mRNA levels in 76 cases were lower than that in adjacent non-cancerous tissues （ 1. 096±1. 110 vs. 3.476±1. 119,t =3. 332,P =0. 017 ; 1. 136±1. 013 vs. 3. 314±1. 105, t = 2. 673, P = 0. 022）. There were close correlations between DKK3 expression and depth of tumor invasion （ P = 0. 006 ）, TNM stage （ P = 0. 005 ） and lymphatic metastasis （P = 0. 009 ）. The expression of DKK3 in gastric cancer was positively related with overall survival rate （47.4% vs. 28.0%, P = 0.011）, as an independent prognosis predictor （P=0.016） . Conclusions DKK3 is an independent prognostic factor of gastric cancer, its decreased expression is significantly related with shortened survival time of gastric cancer patients.

关 键 词：胃肿瘤 病理学 临床 预后 DKK3 

分 类 号：R735.2[医药卫生—肿瘤]