趋化因子CCL2在结直肠癌组织中的表达及其作用机制  

Expression of CCL2 in coiorectal cancer and its role in carcinogenesis

作　　者：张解放 薛洪峰[2] 王茂峰 赵蒙[2] Zhang Jiefang;Xue Hongfeng;Wang Maofeng;Zhao Meng(Department of Gastrointestinal Surgery,Heze Municipal Hospital,Heze 274031,China)

机构地区：[1]菏泽市立医院重症医学科,山东省274031 [2]菏泽市立医院胃肠外科,山东省274031

出　　处：《中华普通外科杂志》2018年第7期589-591,共3页Chinese Journal of General Surgery

摘　　要：目的分析趋化因子CCL2在结直肠癌组织中的表达及其与巨噬细胞相关的机制。方法用Westernblot方法检测17例结直肠癌组织及其对应的癌旁组织中CCL2蛋白表达水平．用流式细胞术分析肿瘤浸润淋巴细胞中巨噬细胞的表型。分离人外周血单核细胞并诱导分化为巨噬细胞，给予一定浓度的CCL2刺激培养12h后，用流式细胞术分析巨噬细胞的表型变化，ELISA方法检测培养上清中VEGF、COX-2和IL-6的表达水平。结果结直肠癌组织中的CCL2蛋白和基因表达水平高于癌旁组织（t=4．017，P〈0．05），并且肿瘤浸润淋巴细胞中巨噬细胞具有相当一部分比例的CCR2表型；CCL2诱导了巨噬细胞CCR2表达的升高（t=5．070，P〈0．05），并且促进了巨噬细胞对VEGF、COX-2和IL-6的分泌作用（均P〈0．05）。结论结直肠癌组织中CCL2表达水平升高，CCL2可能通过招募巨噬细胞和影响其功能发挥肿瘤促进作用。Objective To investigate the expression of CCL2 in colorectal cancer and its carcinogenesis mechanism associated with macrophages. Methods The expression level of CCL2 mRNA in 17 cases of colorectal cancer tissues and corresponding adjacent tissues were analyzed by PCR, and the phenotypes of macrophages in tumor infiltrating lymphocytes （TIL） were analyzed by flow cytometry. Human peripheral blood mononuclear cells were isolated and induced to differentiate into macrophages. After 12 h incubation with CCL2, the phenotypic changes of macrophages were analyzed by flow cytometry. The expression level of VEGF, COX-2 and IL-6 in the supernatant were measured by ELISA assay. Results The expression level of CCL2 in colorectal cancer tissues was significantly higher than that in corresponding adjacent mucosal tissues （ t = 4. 017, P 〈 0. 05 ） , and the macrophages in TIL had a high proportion of CCR2 phenotype. CCL2 was shown to induce increased CCR2 expression in macrophages （t = 5. 070, P 〈 0. 05 ）, and promote the secretion of the tumor growth-associated factors such as VEGF, COX-2 and IL-6 （ all P 〈 0. 05 ）. Conclusion The levels of CCL2 in colorectal cancer were up-regnlated suggesting that CCL2 may play a key role in tumor promotion by recruiting macrophages and influencing their function.

关 键 词：结直肠肿瘤 趋化因子CCL2 巨噬细胞 

分 类 号：R735.34[医药卫生—肿瘤]